压力负荷增高心肌多胺及肥大细胞变化研究  

Changes of myocardial polyamine content and mast cell after chronic pressure overload

作　　者：熊晓兰 贾晓丽 肖超 倪情情 张世忠[1] XIONG Xiaolan;JIA Xiaoli;XIAO Chao;NI Qingqing;ZHANG Shizhong(Medical Collgeg of China Three Gorges University, Yichang, 443002, Chin)

机构地区：[1]三峡大学医学院,湖北宜昌443002

出　　处：《临床心血管病杂志》2018年第4期387-390,共4页Journal of Clinical Cardiology

基　　金：国家自然科学基金(No:30872716);湖北省自然科学基金(No:2015CFB288);湖北省卫生厅科研项目(No:WJ2015MB171);三峡大学科研创新基金(No:SDYC2016086)

摘　　要：目的:探讨慢性压力负荷增高性心肌肥厚大鼠心肌多胺含量及肥大细胞数目的变化规律。方法:通过缩窄大鼠腹主动脉制作压力负荷增高性心肌肥厚动物模型。分别于术后第1、2、3、4、5、6、7和8周称量大鼠体质量、心脏质量,计算心质量指数;采用高效液相法测定心肌多胺含量;采用甲苯胺蓝染色观察心肌肥大细胞数目。结果:与假手术组大鼠比较,手术组大鼠术后各周心指数明显增加(P<0.05),左室心肌的多胺含量明显增高(P<0.05),肥大细胞数量亦明显增多(P<0.05)。结论:慢性心脏压力负荷增高导致心肌肥厚的过程伴有多胺含量增高及肥大细胞数目增多。Objective：To investigate changes of myocardial polyamine content and mast cells count in cardiac hypertrophy induced by chronic pressure overload.Method：Rats of cardiac hypertrophy were induced by partial ligation of abdominal aorta.Body weight and heart weight were weighed at 1,2,3,4,5,6,7 and 8 th week after the operation.Heart weight index was calculated.Myocardial polyamine content was measured by high performance liquid method.The cardiac mast cells count was determined by Toluidine blue staining method.Result：Compared with rats in sham group,the heart weight index,the content of polyamine in myocardium and the count of cardiac mast cells all increased significantly every week after the operation in operation group（all P〈0.05）.Conclusion：The cardiac chronic hypertrophy accompanies with increase of both cardiac polyamine content and mast cells count.

关 键 词：慢性压力负荷过载 心肌重构 多胺 肥大细胞 

分 类 号：R542.2[医药卫生—心血管疾病]