HPLC法测定凹叶景天茎叶中槲皮素、山奈素、异鼠李素的含量  

Determination of Quercetin,Kaempferol and Rhamnolipid in Stem and Leaf of Sedum emarginatum Migo by HPLC

作　　者：丘琴[1] 庾丽峰 陈明伟[1] 甄丹丹[1] 黄丽锦 甄汉深[1] 许玉华[1] QIU Qin;YU Lifeng;CHEN Mingwei;ZHEN Dandan;HUANG Lijing;ZHEN Hanshen;XU Yuhua(Guangxi University of Traditional Chinese Medicine, Nanning Guangxi 530001, China)

机构地区：[1]广西中医药大学,广西南宁530001

出　　处：《广西师范大学学报（自然科学版）》2018年第2期98-104,共7页Journal of Guangxi Normal University:Natural Science Edition

基　　金：广西壮族自治区卫生厅中医药科技专项(GZBZ16-01);壮瑶药协同创新中心项目(桂教科研[2013]20号);广西壮瑶药重点实验室项目(桂科基字[2014]32号);广西重点学科壮药学(桂教科研[2013]16号);广西八桂学者资助项目(J13162);广西高校中药提取纯化与质量分析重点实验室项目(桂教科研[2014]6号)

摘　　要：本文建立了HPLC法同时测定凹叶景天茎叶中槲皮素、山奈素、异鼠李素3个成分含量的方法。采用phenomenon Germini C18(5μm,4.6mm×250mm)色谱柱,流动相甲醇-体积分数0.1%磷酸(体积比55∶45)洗脱,流速1.0mL/min,检测波长370nm,在定量范围内,色谱峰面积与浓度呈良好的线性关系,相关系数均大于0.999,精密度试验、重复性试验和稳定性试验的RSD均小于3%,加样回收率为97.8%~99.1%,所建立的凹叶景天药材的含量测定方法简便、准确、重复性好,可用于凹叶景天药材质量评价。A HPLC method was established for the determination of quercetin, kaempterol and rhamnolipid in the stem and leaf of Sedum emarginatum Migo. The optimized chromatographic conditions were phenomenon Germini C18 （5 μm, 4.6 mm ×250 mm） chromatographic column, methanol -0.1% phosphoric acid （55：45） at a flow rate 1.0 mL/min of the mobile phase with a detection wavelength at 370 nm. The peak area showed good linearity with respect to the concentrations （r〉 0.999）. The RSD of the precision test,repeatability test and stability test were all less than 3%, and the average recovery rates were 97.8%-99.1%.The method was precise,reproducible and stable, which can be applied in the quality control of Sedum emarginatum Migo.

关 键 词：凹叶景天 含量测定 HPLC 槲皮素 山奈素 异鼠李素 

分 类 号：R284.1[医药卫生—中药学]