氰戊菊酯抗性小菜蛾雌成虫对15种植物挥发物的行为反应  

Behavioral Responses of the Female Diamondback Moth,Plutella xylostella(L.)with Fenvalerate Resistent to Fifteen Plant Volatiles

作　　者：田厚军[1,2] 林硕 陈勇[1,2] 陈艺欣 赵建伟[1,2] 魏辉 TIAN Houjun;LIN Shuo;CHEN Yong;CHEN Yixin;ZHAO Jianwei;WEI Hui(Institute of Plant Protection, Fujian Academy of Agricultural Sciences, Fuzhou 350013, China;Fujian Key Laboratory for Mornitoring and Integrated Management of Crop Pests,Fuzhou 350003,China)

机构地区：[1]福建省农业科学院植物保护研究所,福州350013 [2]福建省作物有害生物监测与治理重点实验室,福州350003

出　　处：《西北农业学报》2018年第4期595-601,共7页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：福建省自然科学基金(2016J01140;2015J05061;2015J01116);福建省属公益类科研院所基本科研专项(2015R1024-7;2017R1025-6;2017R1025-9);福建省农科院青年基金(PC2017-10)~~

摘　　要：为了研究小菜蛾对植物挥发物的行为反应与抗性水平之间的关系,采用昆虫Y形嗅觉仪,测定氰戊菊酯抗性和敏感品系小菜蛾雌成虫对15种植物挥发性物质的行为反应。结果表明:抗性品系小菜蛾和敏感品系小菜蛾均对5种绿叶气味(反-2-己烯醛、顺-3-己烯醇、顺-3-己烯乙酸酯、正己醇和正己醛)以及苯甲醛、桉树脑的趋向反应率显著高于石蜡油(P<0.05,χ2检验),表明这7种物质对小菜蛾雌蛾均有一定的诱集作用。在8种萜烯类物质中,敏感小菜蛾雌蛾分别对2种物质(牻牛儿醇、D-柠檬烯)的趋向反应率与石蜡油间无显著差异(P>0.05,χ2检验);而抗性小菜蛾雌蛾分别对4种物质(牻牛儿醇、(R)-(-)-香芹酮、里那醇、(1R)-(+)-α-蒎烯)的趋向反应率与石蜡油均无显著差异。抗性品系小菜蛾停留在Y形管主臂上的比例均少于敏感品系,与敏感品系相比,在较低的氰戊菊酯抗性种群水平下(6.52倍),并没有显著改变小菜蛾雌蛾对寄主植物挥发物的行为反应趋势,但是抗性水平下却加剧小菜蛾趋向反应的比率。In order to study the relationship between behavior responses of diamondback moth[（Plutella xylostella（L.）]to plants volatiles and resistance to fenvalerate,Y-tube olfactormeter was employed to measure behaviour responses of susceptible strain（S strain）and fenvalerate resistant strain（Fen-R strain）of the female moth to fifteen plant volatiles.The results showed that both strains of the moth had significantly higher reaction to five odors of green leaf（trans-2-hexen-1-al,hexanal,1-hexanol,cis-3-hexen-1-ol,cis-3-hexenyl acetate）,benzaldehyde and eucalyptol compared with paraffin（P0.05,χ2-test）,which indicated that these seven matters could trap diamondback moth in some degree.In eight kinds of terpenes,the tendency responses rate of the S strain has no significant difference between two substances（d-limonene and geraniol）and paraffin;and the tendency responses rate of the Fen-R strain had no significant difference between four substances（geraniol,（R）-（-）-carvone,linalool,（-）-α-pinene）and paraffin.The quantitative proportion of the Fen-R strain stayed at the Ytube was less than the S strain.Compared with S strain,under the condition of lower fenvalerate resistance population（6.52 times）,the behavior reaction trend of diamondback moth to host plants volatile matters did not change significantly,but the quantitative proportion of diamondback moth which had taxis reaction increased at the resistant level.Together,these results would provide experimental basis for screening insect sex pheromone synergists.

关 键 词：小菜蛾 植物挥发物 氰戊菊酯抗性 Y形嗅觉仪 

分 类 号：Q965[生物学—昆虫学] Q946.8