外源保幼激素类似物对白蜡虫泌蜡和发育的影响  

Impact of Exogenous Juvenile Hormone Analogue on Wax Excretion and Development of Chinese White Wax Scale(Ericerus pela Chavannes)

作　　者：刘妮[1] 陆沁[1] 张金稳 凌晓霏[1] 刘宏屏 陈航[1] 陈晓鸣[1,3] LIU Ni;LU Qin;ZHANGJin-wen;LING Xiao-fei;LIU Hang-ping;CHEN Hang;CHEN Xiao-ming(Research Institute of Resources lnsects, Chinese Academy of Forestry, Kunming 650224, Yunnan, China;Key LalJoratory of Resources Insects Breeding and Utilization of the State Forestry Administration, Kunming 650224, Yunnan, China;Natural Forest Center of Ynnnan Province, Kunming 650224, Yunnan, China)

机构地区：[1]中国林业科学研究院资源昆虫研究所,云南昆明650224 [2]云南省森林自然中心,云南昆明650224 [3]国家林业局资源昆虫培育与利用重点实验室,云南昆明650224

出　　处：《林业科学研究》2018年第2期114-119,共6页Forest Research

基　　金：中央级公益性科研院所基本科研业务费专项资金重点项目(CAFYBB2017ZB005);国家林业局948项目(2014-4-66)

摘　　要：[目的]探讨外源保幼激素类似物(JHA)对白蜡虫泌蜡和发育的影响。[方法]使用高、中、低浓度保幼激素类似物经喷施、涂干、注射处理白蜡虫2龄雄幼虫。[结果]表明:白蜡虫2龄雄幼虫经外源中等浓度保幼激素类似物(2.5 mg·m L-1)喷施处理可以显著提高白蜡虫2龄雄幼虫的个体泌蜡量,昆明地区增产率平均可达41.50%,峨眉地区增产率平均达25.56%;但此方法在2地区之间表现出差异性,原因可能与两地种虫来源所处的生态环境差异有关。在中、低浓度范围内,JHA对白蜡虫蛹体质量表现出促进作用,高浓度表现出抑制作用;JHA对蛹体长的抑制随着外源保幼激素浓度的增加而增强;外源保幼激素类似物还可以提高白蜡虫蛹的羽化率,羽化率达62.70%~81.62%。[结论]中浓度JHA(2.5 mg·m L-1)喷施处理2龄雄幼虫显著提高白蜡虫泌蜡量;白蜡虫蛹的体长与JHA浓度之间存在一定的负相关性;白蜡虫蛹的体质量表现出低浓度促进,高浓度抑制;喷施和涂干处理,高浓度可显著提高白蜡虫蛹的羽化率;白蜡虫蛹的羽化可能并不受其体质量和体长的影响。[ Objective ] To study the effects of exogenous juvenile hormone analogue （JHA） on Chinese white wax scale with focus on wax secretion and development. [ Method] High, medium and low concentrations of JHA were sprayed on the second-instar male scale, smeared on and injected in the stem of host tree respectively. [ Result] The results of test showed that spraying the male second-instar with medium concentration of JHA （2.5 mg·mL^-1 ） can significantly improve the wax secretion of Chinese white wax scale （ Ericerus pela）, the average production rate in Kunming region reached 41.50%, the average production rate in Emei region reached 25.56%. However, the experimental results show difference between the 2 regions, the reason may be related to the difference of ecological environment in the two areas. For the effects of JHA on pupal development of E. pela, medium and low concentra- tion JHA can promote the weight of pupa, but high concentration showed inhibition ; the inhibition of JHA on pupal length was enhanced with the increase of JHA concentration. JHA can promote pupae eclosion, the eclosion rate could reach as high as 62.70% - 81.62%. [ Conclusion] The treatment of 2nd instar male larvae with medium concentration of JHA could significantly increase the wax secretion of E. pela. There is a negative correlation be- tween the length of pupa and JHA concentration in the E. pela. The body weight of E. pela pupae showed low con- centration promotion and high concentration inhibition; Under spraying and smearing treatments, high concentration can significantly increase the emergence rate of E. pela pupae. The emergence of E. pela pupae is not influenced by its body weight and body length.

关 键 词：白蜡虫 保幼激素类似物 泌蜡量 增产率 羽化率 

分 类 号：S791[农业科学—林木遗传育种]