补阳还五汤对局灶性脑缺血大鼠神经干细胞移植后凋亡相关基因表达的影响  

Effect of Buyang Huanwu decoction on the expression of apoptosis-related genes after neural stem cell transplantation in rats with focal cerebral ischemia

作　　者：廖亮英[1] 蔡光先[2] 周赛男[2] LIAO Liang - ying;CAI Guang - xian;ZHOU Sai - nan(The First Affiliated Hospital of Hunan University of Chinese Medicine,Changsha 410007 ,Hunan, China;Hunan University of Chinese Medicine, Changsha 410208, Hunan, China)

机构地区：[1]湖南中医药大学第一附属医院,湖南长沙410007 [2]湖南中医药大学,湖南长沙410208

出　　处：《湖南中医杂志》2018年第4期132-135,共4页Hunan Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(编号:81302953);湖南省中医药科研计划项目(编号:2014136);湖南省教育厅科研项目(编号:15C1050);中药粉体与创新药物省部共建国家重点实验室培育基地开放基金(编号:ZYFT201510)

摘　　要：目的:探讨补阳还五汤对局灶性脑缺血大鼠神经干细胞移植后凋亡相关基因Bag-1、Bcl-2表达的影响。方法:选取SD大鼠,复制局灶性脑缺血大鼠模型,按神经功能缺损评分进行分层,随机分为模型组、空白移植组、移植组、补阳传统饮片组、补阳超微饮片组,每组15只,分别于细胞移植后7、14、28d观察神经功能评分,采用补阳还五汤超微饮片与传统饮片予以干预,于相应时间点处死大鼠后采用免疫组化法检测凋亡基因Bag-1、Bcl-2的表达。结果:补阳传统饮片组、补阳超微饮片组大鼠神经功能得到明显恢复,Bag-1、Bcl-2蛋白表达上升显著,在各时间点(7、14、28d)与模型组比较差异均有统计学意义(P<0.01或P<0.05);补阳超微饮片组促神经功能积分的恢复及上调Bcl-2、Bag-1蛋白表达作用优于补阳传统饮片组,差异有统计学意义(P<0.01或P<0.05)。结论:补阳还五汤可促进神经功能的恢复,上调Bag-1、Bcl-2蛋白表达,且超微饮片的作用较传统饮片更加显著。Objective： To investigate the effect of Buyang Huanwu decoction on the expression of apoptosis-related genesBag-1 and Bcl-2 after neural stem cell transplantation in rats with focal cerebral ischemia. Methods： Sprague-Dawley rats were selected to establish a rat model of focal cerebral ischemia,and according to the neurologic impairment score,the rats were randomly divided into model group,blank transplantation group,transplantation group,conventional Buyang decoction piece group,and ultrafine Buyang decoction piece group,with 15 rats in each group. Neurological score was determined at 7,14,and 28 days after transplantation,and the ultrafine and conventional decoction pieces of Buyang Huanwu decoction were used for intervention. The rats were sacrificed at these time points,and then immunohistochemistry was performed to measure the expression of apoptosis-related genes Bag-1 and Bcl-2. Results： The conventional Buyang decoction piece group and the ultrafine Buyang decoction piece group had a significant improvement in neurological function and significant increases in the expression of Bag-1 and Bcl-2,and there were significant differences between these two groups and the model group at each time point（at 7,14,and 28 days after transplantation）（P〈0. 01 or P〈0. 05）. Compared with the conventional Buyang decoction piece group,the ultrafine Buyang decoction piece group had a significantly better improvement in neurological score and significantly greater increases in the expression of Bcl-2 and Bag-1（P〈0. 01 or P〈0. 05）. Conclusion： Buyang Huanwu decoction can promote the recovery of neurological function and upregulate the expression of Bag-1 and Bcl-2 and has a significantly better effect than conventional decoction pieces.

关 键 词：局灶性脑缺血 神经干细胞 细胞移植 补阳还五汤 BAG-1 Bcl-2 实验研究 

分 类 号：R285.5[医药卫生—中药学]