补肾生血方对肾性贫血大鼠EPO水平及EPORmRNA的影响  

Effect of Bushen Shengxue prescription on the mRNA expression of erythropoietin and erythropoietin receptor in rats with renal anemia

作　　者：范军[1] 原洋 郭晓红 范高伟 车树强 FAN Jun;YUAN Yang;GUO Xiao - hong;FAN Gao - wei;CHE Shu - qiang(Affiliated Hospital of Tianjin Academy of Traditional Chinese Medicine ,Tianjin 300120, China;Tianjin University of Traditional Chinese Medicine,Tianjin 300193, China)

机构地区：[1]天津市中医药研究院附属医院,天津300120 [2]天津中医药大学,天津300193

出　　处：《湖南中医杂志》2018年第4期138-141,共4页Hunan Journal of Traditional Chinese Medicine

基　　金：天津市中医药管理局中医中西医结合科研课题(编号:2015008)

摘　　要：目的:研究补肾生血方对肾性贫血大鼠促红细胞生成素(EPO)及EPOR mRNA水平的影响。方法:Wistar大鼠50只,分为对照组、模型组、中药组、EPO组、中药+EPO组,每组各10只,对除对照组外的其余组大鼠通过腺嘌呤灌胃制备肾性贫血大鼠模型,分别给予相应药物进行干预。分别于给药第2、8周观察血常规指标(Hb、RBC、HCT)、肾功能指标(BUN、Scr)的水平及EPO浓度,给药8周后处死大鼠,提取骨髓单个核细胞,测定EPOR mRNA表达量的变化。结果:与治疗2周相比,治疗8周中药组、EPO组、中药+EPO组大鼠的BUN、Scr显著降低,Hb、RBC、HCT、EPO水平及EPOR mRNA表达量显著上升,差异均有统计学意义(P<0.05)。结论:补肾生血方具有改善肾性贫血模型大鼠肾功能、肾性贫血状况,其机制可能与提高骨髓有核细胞EPOR mRNA表达量,减轻毒素对EPOR的抑制,加强对内源性EPO反应性有关。Objective： To investigate the effect of Bushen Shengxue prescription on the mRNA expression of erythropoietin（EPO） and erythropoietin receptor（EPOR） in rats with renal anemia. Methods： A total of 50 Wistar rats were divided into control group,model group,traditional Chinese medicine（TCM） group,EPO group,and TCM ＋ EPO group,with 10 rats in each group. All rats except those in the control group were given adenine by gavage to establish a rat model of renal anemia,and related drugs were given as intervention. Routine blood indices[hemoglobin（Hb）,red blood cell count（RBC）,and hematocrit（HCT） ],renal functional markers [blood urea nitrogen（BUN） and serum creatinine（Scr） ],and level of EPO were measured at weeks2 and 8 of treatment. The rats were sacrificed after 8 weeks of administration,bone marrow mononuclear cells were extracted,and the change in the mRNA expression of EPOR was measured. Results： The TCM group,the EPO group,and the TCM ＋ EPO group had significant reductions in BUN and Scr and significant increases in Hb,RBC,HCT,EPO level,and mRNA expression of EPOR from week 2 to week 8 of treatment（P〈0. 05）. Conclusion： Bushen Shengxue prescription can significantly improve renal function and the condition of renal anemia in rats with renal anemia,possibly by improving the mRNA expression of EPOR in bone marrow nucleated cells,reducing the inhibition of EPOR by toxins,and enhancing the reactivity of endogenous EPO.

关 键 词：肾性贫血 补肾生血方 EPO EPOR mRNA 实验研究 

分 类 号：R285.5[医药卫生—中药学]