复创产品指纹图谱的比较研究  

A comparative study of fingerprints of wound-healing products

作　　者：易延逵[1] 陈亚芬 李钰莹 卢静雅 薛雅琪 YI Yan- kui;CHEN Ya - fen;LI Yu - ying;LU Jing - ya;XUE Ya - qi(School of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, Guangdong, China)

机构地区：[1]南方医科大学中医药学院,广东广州510515

出　　处：《湖南中医杂志》2018年第4期152-156,共5页Hunan Journal of Traditional Chinese Medicine

基　　金：广东省中医药管理局建设中医药强省科研课题(编号:20151298)

摘　　要：目的:对复创产品指纹图谱进行研究,比较分析不同工艺制备的复创产品中苷类成分指纹图谱的差异。方法:采用HPLC法,以人参皂苷Rg1为参照物,C18色谱柱,乙腈-水为流动相梯度洗脱,流速1.00m L/min,柱温25℃,检测波长203nm,进样10μL,记录时间75min,以人参皂苷Rg1色谱峰(S峰)的保留时间和峰面积为1计算各峰相对保留时间和相对峰面积比值,并进行方法学考查;采用中国国家药典委员会制定的"中药色谱指纹图谱相似性评价系统"进行相似度评价。结果:制定了不同工艺制备的复创产品中苷类成分指纹图谱,标注了复创产品中苷类成分8个共有指纹峰。不同工艺制备的复创产品各共有指纹峰的相对保留时间比较接近,而各共有指纹峰的相对峰面积则相差较大。结论:通过对复创产品中苷类成分指纹图谱的制定和比较分析,为指纹图谱的制定提供了实验依据,对提高天然产物质量控制技术进行了有意义的探索。Objective： To investigate the fingerprints of wound-healing products and the differences in the fingerprints of glycosides in wound-healing products manufactured by different processes. Methods： With ginsenoside Rg1 as a reference,high-performance liquid chromatography was performed on a C18 column with a mobile phase of acetonitrile-water for gradient elution at a flow rate of 1. 00 m L/min,a column temperature of 25℃,a detection wavelength of 203 nm,a sample size of 10μL,and a recording time of 75 min. With the retention time and peak area of ginsenoside Rg1 chromatographic peak as 1,the relative retention time and the relative peak area were calculated,and a methodological examination was also performed. The similarity of fingerprints was evaluated based on＂the similarity evaluation system for chromatographic fingerprints of Chinese materia medica＂developed by Chinese Pharmacopoeia Commission. Results： The fingerprints of glycosides in wound-healing products manufactured by different processes were established,and 8 common fingerprint peaks were labeled. These common fingerprint peaks had a similar retention time,while there was a significant difference in relative peak area between these common fingerprint peaks. Conclusion： The establishment and comparative analysis of the fingerprints of glycosides in wound-healing products provide an experimental basis for establishing fingerprints and are meaningful to improving the quality control technique of natural products.

关 键 词：HPLC法 指纹图谱 人参皂苷RG1 复创产品 

分 类 号：R284.1[医药卫生—中药学]