蜈蚣油对中国白兔深Ⅱ度烧伤组织中VEGF、IL-8表达水平的影响  

Research on influence of centipede oil on VEGF and il-8 expression levels in deep II scald tissues in Chinese rabbit

作　　者：杨媚月 黄湘俊 刘秀芝[2] 夏传林[2] Yang Meiyue;Huang Xiangjun;Liu Xiuzhi(The First Hospital of Hunan University of Chinese Medicine, Hunan 410007 China)

机构地区：[1]湖南中医药大学第一附属医院 [2]湖南中医药大学

出　　处：《护理研究》2018年第7期1078-1081,共4页Chinese Nursing Research

基　　金：2017年度湖南省中医药科研计划项目;编号:201781

摘　　要：[目的]通过检测蜈蚣油对白兔深Ⅱ度烫伤创面组织中血管内皮细胞生长因子(VEGF)、白细胞介素-8(IL-8)表达水平的影响,初步揭示蜈蚣油减轻烫伤创面水肿、促进愈合的作用机制。[方法]将45只深Ⅱ度烫伤创面的白兔随机分3组,观察组给予经验方蜈蚣油外敷,对照组用湿润烧伤膏外敷,模型组仅造成白兔深Ⅱ度烫伤、创面不做任何处理。在烫伤即刻及烫后第1天、第3天、第5天、第7天5个时点进行创面形态学观察,病理取样烫伤前、烫伤造模成功后、用药7d后进行创面组织HE染色及免疫组化VEGF、IL-8检测。[结果]烫伤第7天各组间创面面积比较,差异有统计学意义(P<0.05);与模型组比较,观察组和对照组创面组织VEGF表达水平明显升高,差异有统计学意义(P<0.05);IL-8表达水平明显降低,差异有统计学意义(P<0.05)。[结论]蜈蚣油可促进创面组织VEGF表达、减少IL-8表达,从而减轻深Ⅱ度烫伤创面水肿和炎症反应,促进创面的愈合。Objective：To detect the effect of centipede oil on Vascular endothelial growth factor（VEGF）and Interleukin-8interleukin 8（IL-8）expression level in deep II scald wound surface tissues of rabbit,in order to reveal its mechanism on reducing scald wound surface edema and promoting healing.Methods：A total of 45rabbits with deep II scald wound surface were randomly divided into 3groups,the observation group using centipede oil for external application,the control group using moist burn ointment for external application;the model group with no processing.Wound morphology observation was conducted at the moment of scald,1day,3days,5days,and 7days after scalding.Pathological sampling was conducted before the scald,after the scald,and 7days after medication on wound tissue HE staining and immunohistochemical VEGF and il-8were detected.Results：There was a statistically significant difference in the wound healing areas on the 7th day of scald among all groups（P〈0.05）.Compared with the model group,the expression level of VEGF significantly increased in the observation group and control group,and the difference was statistically significant（P〈0.05）.The expression level of IL-8was significantly reduced and the difference was statistically significant（P〈0.05）.Conclusions：The centipede oil could reduce the deepⅡscald wound surface edema and inflammation reaction by improving the expression level of wound tissue VEGF and reducing the expression level of IL-8,in order to promote wound healing.

关 键 词：蜈蚣油 深Ⅱ度烫伤 血管内皮细胞生长因子 白细胞介素-8 实验研究 

分 类 号：R47[医药卫生—护理学]