蝌蚪状类弹性蛋白多肽相变特性分析  

Phase Transition Behavior of Tadpole-Like Elastin-Like Polypeptides

作　　者：邱岳 张丹丹 吴姝羽 蔡征文 林源清[1] 张光亚[1] QIU Yue;ZHANG Dandan;WU Shuyu;CAI Zhengwen;LIN Yuanqing;ZHANG Guangya(College of Chemical Engineering, Iluaqiao University, Xiamen 361021, China)

机构地区：[1]华侨大学化工学院,福建厦门361021

出　　处：《华侨大学学报（自然科学版）》2018年第3期414-419,共6页Journal of Huaqiao University(Natural Science)

基　　金：国家自然科学基金资助项目(21376103);福建省自然科学基金资助项目(2017J01065)

摘　　要：为研究不同盐类型及浓度对蝌蚪状ELPs_(40)相变特性的影响,探究霍夫曼斯特(Hofmeister)离子序中阴、阳离子对蝌蚪状ELPs_(40)相变温度的影响,通过设计、表达和纯化后,获得由谍标签/捕谍器(SpyTag/SpyCatcher),以及由40个重复的线性类弹性蛋白多肽所形成的2个蝌蚪型结构的ELPs_(40).结果表明:蝌蚪状ELPs_(40)相变温度比ELPs_(80)相变温度高1.35℃;阴离子对蝌蚪状ELPs_(40)相变温度的影响符合Hofmeister离子序现象,但NH4^+,K^+,Na^+等阳离子却发生逆Hofmeister离子序现象;在NaCl,Na_2SO_4,NaNO_3,K_2SO_4,KCl,KNO_3,NH_4Cl,NH_4NO_3,(NH_4)_2SO_4等不同浓度缓冲液中,蝌蚪状ELPs_(40)发生相变所需的温度范围显著高于线性ELPs_(40),这与其特殊的结构密切相关.In order to study the effects of different salt types and concentrations on the phase transition characteristics of elastin-like polypeptides 40（ELPs40）,the influence of the anions and cations in the Hofmeister on the phase transition temperature of ELPs40 was investigated.After designing,expressing and purifying,the ELPs40 with the SpyTag/SpyCatcher and two 40-repetitive linear elastin-like peptides were obtained.The results show that the phase transition temperature of ELPs40 is higher with 1.35℃ than that of the ELPs80.The effect of anions on the phase transition temperature of ELPs40 accords with Hofmeister,but the cations such as NH4＋,K＋,Na＋ have inverse Hofmeister;in the buffers with different concentrations such as NaCl,Na2SO4,NaNO3,K2SO4,KCl,KNO3,NH4Cl,NH4NO3,（NH4）2SO4,etc.,temperature range required for phase transition of the tadpole-like ELPs40 is significantly higher than that of the linear ELPs40,which should be closely related to its special structure.

关 键 词：蛋白拓扑结构 蝌蚪状ELPs40 相变温度 Hofmeister离子序 构效关系 

分 类 号：Q35[生物学—遗传学]