利用CRISPR/Cas9系统定向编辑水稻SD1基因  

Targeted Editing of Rice SD1 Gene Using CRISPR/Cas9 System

作　　者：胡雪娇 杨佳 程灿[1] 周继华[1] 牛付安[1] 王新其[1] 张美良[2] 曹黎明[1] 储黄伟[1] HU Xuejiao;YANG Jia;CHENG Can;ZHOU Jihua;NIU Fuan;WANG Xinqi;ZHANG Meiliang;CAO Liming;CHU Huangwei(Institute of Crop Breeding and Cultivation, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China;School of Agricultural Sciences, Jiangxi Agricultural University, Nanchang 330045, China;College of Food Sciences ＆ Technology, Shanghai Ocean University, Shanghai 201306, China)

机构地区：[1]上海市农业科学院作物育种栽培研究所,上海201403 [2]江西农业大学农学院,南昌330045 [3]上海海洋大学食品学院,上海201306

出　　处：《中国水稻科学》2018年第3期219-225,共7页Chinese Journal of Rice Science

基　　金：上海市科技兴农重点攻关计划资助项目(沪农科攻字2014第7-1-2号);上海市浦江人才计划资助项目(16PJ1408900);上海市种业发展计划资助项目(沪农科种字2016第1-3号)

摘　　要：【目的】半矮秆水稻品种的选育和应用是水稻育种的最重大成果之一。半矮秆品种大多是半矮秆基因SD1(semi-dwarf1)功能缺失突变体,为了获得sd1突变体,本研究对SD1基因进行了定向编辑。【方法】利用CRISPR/Cas9系统,以SD1基因为靶基因,构建基因编辑载体CRISPR-SD1,用农杆菌介导的方法转化水稻恢复系申繁17和申繁24。【结果】在2个转化受体的T_0代均获得了纯合的sd1突变体,并且在T_1代株系中分离出了不含转基因序列的植株。2个品种的sd1突变体与各自的野生型相比,株高分别下降了25%左右。【结论】利用CRISPR/Cas9系统可以有效地对目的基因进行编辑,在水稻分子育种领域具有巨大的应用价值。【Objective】In the last century, the usage of semi-dwarf rice varieties brought about significant improvements and great achievements in rice breeding. Most of the semi-dwarf rice varieties have loss-of-function mutations in the Semi-Dwarf1 （SD1） gene. In order to obtain sd1 mutant, targeted editing of SD1 gene was performed. 【Method】SD1 gene editing vector CRISPR-SD1 was constructed by using CRISPR/Cas9 system, and transformed into two rice restore lines Shenfan 17 and Shenfan 24 by the Agrobacterium-mediated method.【Result】 Homozygous sd1 mutants were obtained in T0 generation in both varieties, and vector-free sd1 mutant lines were segregated from the T1 population. The plant heights of the two sd1 mutants were decreased by 25% as compared with those of their wild types. 【Conclusion】CRISPR/Cas9 is a powerful tool for rice target gene editing, and has enormous potential in rice molecular breeding.

关 键 词：CRISPR/Cas9 基因编辑 水稻 半矮秆基因 

分 类 号：Q755[生物学—分子生物学] S511.032[农业科学—作物学]