NKG2D基因多态性与云南汉族人群2型糖尿病的相关性  

The Association of Polymorphisms in NKG2D Gene with T2DM in Chinese Han Population in Yunnan Province

作　　者：洪超[1] 陶文玉[2] 周紫云 杨曼[2] 赵雨笛 赵凎 王晓苓[2] 陈珺 刘楠楠 史荔[1] 李奕平[2] HONG Chao;TAO Wenyu;ZHOU Ziyun;YANG Man;ZHAO Yudi;ZHAO Gan;WANG Xiaoling;CHEN Jun;LIU Nannan;SHI Li;LI Yiping(Institute of Medical Biology,Chinese Academy of Medical Sciences ＆ Peking Union Medical College,Kunming 650118,Yunnan,China;Department of Endocrinology and Metabolism,The Second People＇s Hospital of Yunnan Province ＆ The First Hospital of Yunnan University ＆ The Fourth Affiliated Hospital of Kunming Medical University,Kunming 650021,Yunnan,China;School of Life Science,Y-unnan University,Kunming 650500,Yunnan,China)

机构地区：[1]中国医学科学院&北京协和医学院医学生物学研究所,云南昆明650118 [2]云南省第二人民医院&云南大学第一医院&昆明医科大学第四附属医院内分泌代谢科,云南昆明650021 [3]云南大学生命科学学院,云南昆明650500

出　　处：《贵州医科大学学报》2018年第5期513-516,521,共5页Journal of Guizhou Medical University

基　　金：国家自然科学基金项目(31660313和30960148);云南省科技厅-昆明医科大学应用基础研究联合专项(2013FB181)

摘　　要：目的:研究NKG2D基因中的多态性位点与云南汉族人群2型糖尿病(T2DM)的易感性的关系。方法:采用Taq Man基因分型的方法对343例T2DM患者和325例健康对照人群的NKG2D基因多态性位点(rs2255336和rs2617160)进行基因分型,分析NKG2D基因多态性位点的等位基因和基因型在T2DM组和对照组人群中的分布差异;构建2个多态性位点的单倍型,分析各单倍型在2组中的分布差异。结果:结果显示,rs2255336等位基因在2组被检者中的分布频率比较,差异无统计学意义(P>0.05),rs2617160等位基因在2组被检者的分布频率比较,差异有统计学意义(P=0.030),该位点等位基因A在对照组中的分布频率显著高于对照组,可能是云南汉族人群T2DM的保护性因素(OR=0.79;95%CI为0.64~0.98)。结论:NKG2D基因中的多态性位点rs2617160等位基因A可能是云南汉族人群T2DM的保护性因素。Objective： To investigate the association of SNP（ single nucleotide polymorphisms） in NKG2D gene with type 2 diabetes mellitus（ T2DM） in Yunnan Han population. Methods： The polymorphisms of NKG2D（ rs2255336 and rs2617160） were genotyped by using Taqman assay in 343 T2DM patients and 325 healthy people. The allelic and genotypic frequencies of the SNP in T2DM group and control group were analyzed. Additionally,the haplotypes of two polymorphisms were constructed and the association of the haplotypes with T2DM group and control group was analyzed.Results： The results showed that the allelic frequency of rs2617160 allele A was significantly higher in control group than T2DM groups（ P = 0. 030,OR = 0. 79; 95% CI： 0. 64 - 0. 98） likely due to the protective factor of T2DM in Chinese Han population in Yunnan province. However there was no significant difference of the distribution for rs2255336 between T2DM and control group（ P〉0. 05）. Conclusion： Results indicated that the allele A of rs2617160 in NKG2D gene might be the protective factor of T2DM in Chinese Han population in Yunnan province.

关 键 词：2型糖尿病 炎症反应 NK细胞 NKG2D基因 多态性 云南 汉族 相关性分析 

分 类 号：R587.1[医药卫生—内分泌]