骨康胶囊对SaOS-2人成骨样细胞增殖、分化及矿化的影响  

Effects of Gukang Capsule on Cell Proliferation,Differentiation and Mineralization of Human Osteoblast-like SaOS-2 Cells

作　　者：杨健[1,2] 陆定艳 彭潇 董莉 沈艳珍[4] 李勇军 席晓岚[1,2] 刘亭[1] YANG Jian;LU Dingyan;PENG Xiao;DONC Li;SHEN Yanzhen;LI Yongjun;XI Xiaolan;LIU Ting(Guizhou Provincial Key Laboratory of Pharmaceutics / State Key Laboratory of Functions and Applications of Medicinal Plants,Guizhou Medical Ukliversity,Guiyang 550004,Guizhou,China;School of Pharmacy,Guizhou Medical University,Guiyang 550025,Guizhou,China;Engineering Research Center for the Development and Application of Ethnic Medicine and TCM（Ministry of Education）,Guizhou Medical University,Guiyang 550004,Guizhou,China;Guizhou Weikang Zifan Pharmaceutical Co.,Ltd,Xiuwen 550200,Guizhou,China)

机构地区：[1]贵州医科大学贵州省药物制剂重点实验室/药用植物功效与利用国家重点实验室,贵州贵阳550004 [2]贵州医科大学药学院,贵州贵阳550025 [3]贵州医科大学民族药与中药开发应用教育部工程研究中心,贵州贵阳550004 [4]贵州维康子帆药业股份有限公司,贵州修文550200

出　　处：《贵州医科大学学报》2018年第5期517-521,共5页Journal of Guizhou Medical University

基　　金：贵州省科技计划项目[黔科合J重大字(2015)2002;黔科合LH字(2016)7373];贵州省教育厅项目[黔教合重大专项字(2015)040];贵州省创新人才团队项目[黔科合平台人才(2016)5613;黔科合平台人才(2016)5677]

摘　　要：目的:研究骨康胶囊对体外培养的SaOS-2成骨肉瘤细胞增殖、分化及矿化的影响。方法:培养成骨肉瘤细胞SaOS-2,设立对照组、不同浓度的骨康胶囊组(生药浓度为1、10、100、200、400、800 mg/L)及维生素D3组,采用MTT法检测SaOS-2细胞增殖能力;选择10、100及200 mg/L骨康胶囊浓度处理SaOS-2细胞,对-硝基苯磷酸盐法(PNPP法)检测SaOS-2细胞碱性磷酸酶(ALP)活性,ELISA法检测SaOS-2细胞骨钙素(OTC)和钙离子(Ca^(2+))分泌能力,采用细胞钙茜素红染色法对成骨细胞进行染色、观察SaOS-2细胞矿化能力。结果:与对照组相比,骨康胶囊在体外可以显著促进成骨肉瘤细胞的增殖(P<0.01),当骨康胶囊浓度为200 mg/L时作用最大,此后随着骨康胶囊浓度增大对SaOS-2细胞增殖的促进作用有明显减弱的趋势;在10、100、200 mg/L浓度范围内,骨康胶囊剂量依赖性地增加SaOS-2细胞ALP活性、促进OTC和Ca^(2+)的分泌及细胞矿化结节形成(P<0.05或P<0.01)。结论:骨康胶囊对体外培养的SaOS-2细胞的增殖、分化和矿化有明显的促进作用。Objective： To study the effects of Gukang capsule on cell proliferation,differentiation and mineralization of human osteoblast-like SaOS-2 cells cultivated in vitro. Methods： SaOS-2 cells were cultivated. The control group,Gukang capsule group with different concentrations（ The biological concentrations were 1,10,100,200,400 and 800 mg/L respectively） and Vitamin D3 group were set up,and cell proliferation was tested by MTT assay. The SaOS-2 cells were treated with the Gukang capsule（ 10,100 and 200 mg/L respectively）,and the Alkaline phosphatase（ ALP） activity was measured by PNPP method. Secretion of osteocalcin（ OTC） and Ca^2＋was detected by ELISA assay.Osteoblasts were stained with calcium alizarin red staining method and mineralization of SaOS-2 cells was observed. Results： Compared with the control group,Gukang capsule could significantly promote proliferation of SaOS-2 cells in vitro（ P〈0. 01）,and increase ALP activity in a dose-dependent manner（ P〈0. 01）. Moreover,it could also increase the secretion of OTC and Ca^2＋（ P〈0. 05,P〈0. 01）,and significantly promote the formation of osteoblast mineralization nodules（ P〈0. 05）.Conclusions： Gukang capsule could increase cell proliferation,differentiation and mineralization activities in SaOS-2 cells cultivated in vitro,which may contribute to its therapeutic effects.

关 键 词：骨质疏松症 骨康胶囊 成骨肉瘤细胞 增殖 分化 矿化 碱性磷酸酶 细胞骨钙素 

分 类 号：R285[医药卫生—中药学] R738.1[医药卫生—中医学]