基于平面波导传感器的恩诺沙星与诺氟沙星同时检测方法  

Simultaneous detection of enrofloxacin and norfloxacin by planar waveguide immunosensor

作　　者：李树莹 田艳 陈蓓 宋保栋[1] 刘丽[1] 何苗[1] LI Shuying;TIAN Yan;CHEN Bei;SONG Baodong;LIU Li;HE Miao(State Key Joint Laboratory of Environmental Simulate and Pollution Control, Center for Sensor Technology of Environment and Health, School of Environment, Tsinghua University, Beijing 100084;Guangxi Environmental Monitoring Centre, Nanning 530028)

机构地区：[1]清华大学环境学院,环境模拟与污染控制国家联合重点实验室,清华大学环境与健康传感技术研究中心,北京100084 [2]广西壮族自治区环境监测中心站,南宁530028

出　　处：《环境科学学报》2018年第5期1899-1905,共7页Acta Scientiae Circumstantiae

基　　金：广西水环境高风险污染物快速监测新技术开发及示范应用项目(No.桂科AB17129007)

摘　　要：为了实现对于环境中抗生素恩诺沙星与诺氟沙星的同时快速灵敏检测,本研究基于间接竞争免疫反应模式及荧光全内反射原理,以本课题组自主研发的平面波导生物传感器为平台,建立了恩诺沙星和诺氟沙星的同时快速检测方法.通过对抗体浓度、pH及钙离子浓度的优化,提高了检测方法的灵敏度,结果表明,恩诺沙星的检测限为0.34μg·L^(-1),诺氟沙星的检测限为0.14μg·L^(-1).在单物质检测的基础上,首次实现了基于平面波导传感器的两种抗生素同时检测,检测周期仅15 min,检测限为0.06μg·L^(-1).本研究从理论上分析了同时检测时检测限下降、灵敏度提高的原因,为利用生物传感器法同时检测抗生素提供了理论指导及技术支撑.To meet the demand of the simultaneous and rapid detection of enrofloxacin and norfloxacin in the environment,a rapid and sensitive method was successfully established by planar waveguide immunosensor,which was based on the indirect competitive immunoassay and total internal reflect fluorescent. And the planar waveguide immunosensor platform was developed by our research group. After optimization of the antibody concentration,p H and calcium ion concentration,a detection limit at 0.34 μg·L-1（S/N= 3） of enrofloxacin and 0.14 μg·L^（-1）（S/N= 3） of norfloxacin was achieved. On the basis of single matter detection method,a simultaneous detection of enrofloxacin and norfloxacin method was established. The detection limit for both substances dropped to 0.06 μg·L^（-1）,the reasons for the sensitivity improvement were analyzed. This study provided theoretical guidance and technical support for the simulataneous detection of antibiotics.

关 键 词：恩诺沙星 诺氟沙星 平面波导生物传感器 同时检测 环境 

分 类 号：X83[环境科学与工程—环境工程]