牛子宫内膜上皮细胞的体外培养及鉴定  

Culture and Identification of Bovine Endometrial Epithelial Cell

作　　者：詹同彤 杨健[1] 蔡德霖 王相国[1] 梁鸿斌[1] 谷传慧 倪和民[1] 郭勇[1] Zhan Tongtong;Yang Jian;Cai Delin;Wang Xiangguo;Liang Hongbin;Gu Chuanhui;Ni Hemin;Guo Yong(Animal Science and Technology College, Beijing University of Agriculture, Beijing 102206)

机构地区：[1]北京农学院动物科学技术学院,北京102206

出　　处：《中国农学通报》2018年第11期118-122,共5页Chinese Agricultural Science Bulletin

基　　金：2013年度国家自然基金面上项目"牛体内外植入前胚胎IFN-τ的差异表达及对子宫上皮细胞ISG15和Wnt7a表达的体外诱导"(项目批准号:31272526);2016年度科技创新服务能力建设-科技计划重点项目"牛胚胎源IFN-τ及其受体体外调节子宫源Integrinανβ3差异表达的研究"(项目批准号:PXM2016_014207_000064)

摘　　要：旨在改良牛子宫内膜细胞原代培养方法,并鉴定其特征。分别比较了单纯组织块培养法和先组织块培养后再消化纯化的方法对牛子宫内膜细胞进行培养以及纯化。经免疫荧光染色方法对两种方法获得的牛子宫内膜上皮细胞进行角蛋白表达鉴定。研究结果表明:先组织块培养后再消化纯化的方法获得的牛子宫内膜上皮细胞形态良好,纯度较高。因此,组织块培养后再消化纯化得到的牛子宫内膜上皮细胞是一种操作简单、高效的方法,用该方法得到的细胞可以在体外传至4~5代,此结果为牛繁殖生理及其机制模型的建立奠定基础。The aim of this study is to improve the primary culture method of bovine endometrial epithelial cells and to identify their characteristics. Tissue block culture method as well as digestion and purification after tissue block culture method were applied respectively to isolate and culture bovine endometrial epithelial cells. The expression of cytokeratin in epithelial cells was identified by immunofluorescent staining. The results showed that digestion and purification after tissue block culture could acquire high percentage of live ceils and high purity of cells. Therefore, this method is easy and repeatable for culture of bovine endometrial epithelial cells in vitro. The cells obtained by this method could be sub-cultured for 4-5 generations in vitro. This method establishes a foundation for research of physiology and mechanism of bovine reproduction.

关 键 词：牛 子宫内膜上皮细胞 组织块培养法 纯化 

分 类 号：S814.8[农业科学—畜牧学]