玉米自交系苗期耐盐性鉴定及筛选研究  

Salt-tolerance Identification and Screening of Maize Inbred Lines at Seedling Stage

作　　者：王明泉[1,2] 李春霞[1] 龚士琛[1] 闫淑琴[1] 李国良[1] 扈光辉[1] 苏俊[1] 任洪雷[1] 杨剑飞[1] 张翼飞[2] 杨克军[2] Wang Mingquan;Li Chunxia;Gong Shichen;Yan Shuqin;Li Guoliang;Hu Guanghui;Su Jun;Ren Honglei;Yang Jianfei;Zhang Yifei;Yang Kejun(Maize Research Institute of Heilongfiang Academy of Agricultural Sciences, Harbin 150086;College of Agronomy, Heilongjiang Bayi Agricultural University, Daqing Heilongjiang 163319)

机构地区：[1]黑龙江省农业科学院玉米研究所,哈尔滨150086 [2]黑龙江八一农垦大学农学院,黑龙江大庆163319

出　　处：《中国农学通报》2018年第12期30-35,共6页Chinese Agricultural Science Bulletin

基　　金：国家重点研发计划"玉米种质资源精准鉴定与创新利用"(2016YFD0100103);国家重点研发计划"东北早熟区玉米种质改良与强优势杂交种创制"(2016YFD0101202);国家重点研发计划"东北北部春玉米;粳稻水热优化配置丰产增效关键技术研究与模式构建"(2017YFD0300506);黑龙江省农业科学院创新项目"北方玉米种质资源耐盐性评价及部分功能基因分析"(2014ZD003);黑龙江省重大招标项目"早熟;适宜机械化收获玉米种质资源创新及新品种选育"(GA14B103)

摘　　要：旨在加强玉米耐盐性的研究和利用,本研究采用水培法,以NaCl胁迫拟田间盐生条件,在适宜的盐胁迫处理浓度的基础上,以耐盐系数为指标,通过主成分分析、隶属函数分析和聚类分析对242份玉米自交系苗期的耐盐性进行综合鉴定。结果表明:发芽率、根干重、苗干重、根鲜重、根长和苗鲜重可作为玉米自交系的苗期耐盐性筛选和鉴定指标;通过聚类分析可将242份玉米自交系苗期的耐盐性划分为5类,其中B红-2等12份玉米自交系的耐盐性较强,NC320等42份玉米自交系的耐盐性中等,50K-4-1等58份玉米自交系的耐盐性一般,H070480等77份玉米自交系的耐盐性敏感,甘1号等53份玉米自交系的耐盐性高度敏感。To enhance the research and application of maize salt tolerance, we use hydroponics with NaCl stress simulating salt condition in field. Based on appropriate Na Cl stress concentrations, and taking the salttolerance coefficients as indexes, we identified the salt tolerance of 242 maize inbred lines at seedling stagethrough principal component analysis, membership function analysis and cluster analysis. The results showedthat, the germination rate, root dry weight, seedlings＇ dry weight, root fresh weight, root length, and seedlings＇ fresh weight could be used as indicators for screening and identifying salt-tolerant maize inbred lines atseedling stage. With clustering analysis, 242 maize inbred lines at seedling stage were classified into fivegroups： 12 were high salt-tolerance, like B hong-2; 42 were medium salt-tolerance, like NC320; 58 weregeneral salt-tolerance, like 50 K-4-1; 77 were salt-sensitive, like H070480; and 53 were highly salt-sensitive,like Gan 1.

关 键 词：NACL胁迫 玉米自交系 苗期 耐盐性 综合评价 

分 类 号：S513[农业科学—作物学]