矢车菊素与矢车菊素-3-O-葡萄糖苷对H2O2诱导HepG-2细胞氧化应激保护作用的比较  

Comparison of protective effects of cyanidin and cyanidin-3-O-glucoside on oxidative stress induced by H_2O_2 in HepG-2 cell model

作　　者：张楚阳 张珂嘉 刘琴 ZHANG Chuyang;ZHANG Kejia;LIU Qin(Key Laboratory of Grains and Oils Quality Control and Processing,Collaborative Innovation Center for Modern Grain Circulation and Safety,College of Food Science and Engineering,Nanjing University of Finance and Economics, Nanjing 210023, China)

机构地区：[1]南京财经大学食品科学与工程学院江苏省现代粮食流通与安全协同创新中心江苏高校粮油质量安全控制及深加工重点实验室,江苏南京210023

出　　处：《南京工业大学学报（自然科学版）》2018年第3期31-37,共7页Journal of Nanjing Tech University(Natural Science Edition)

基　　金：江苏省高校自然科学研究重大项目(11KJA550001);江苏高校优势学科建设工程

摘　　要：以人肝癌细胞HepG-2为模型,以细胞抗氧化水平为指标,比较研究一种花色苷元矢车菊素(Cy)和其对应的花色苷矢车菊素-3-O-葡萄糖苷(C3G)对H_2O_2诱导的细胞氧化应激的保护作用。采用噻唑蓝(MTT)法检测细胞的存活率,采用总抗氧化能力(T-AOC)和细胞中的超氧化物歧化酶(SOD)活力、谷胱甘肽(GSH)及丙二醛(MDA)的含量评价样品的抗氧化保护作用。结果表明:两种花青素在0~0.5 mmol/L范围内对HepG-2细胞无明显的毒性;与H_2O_2氧化应激模型组相比,在高(400μmol/L)、中(200μmol/L)、低(50μmol/L)3种浓度下两种结构的花青素处理均可显著提高细胞总抗氧化能力、超氧化物歧化酶活力和还原型谷胱甘肽含量,降低细胞内丙二醛含量,抗氧化能力与花青素浓度呈正相关性;而Cy的细胞抗氧化作用显著高于C3G。该研究结果为阐明花青素的构效关系提供了体外研究的新证据。Protection effects of cyanidin（ Cy） and its derivate cyanidin-3-O-glucoside（ C3G） on cellular oxidative stress induced by H2O2 on HepG-2 cell line were compared.The cell viabilities were detected by thiazolyl blue tetrazolium bromide（ MTT） assay and the antioxidant protection were evaluated by total antioxidant capacity（ T-AOC）,the activity of superoxide dismutase（ SOD）,concentration of glutathione（GSH） and malondialdehyde（MDA） in cell.Results showed that two anthocyanins had no significant toxic effects on cultured HepG-2 cells under the concentration range of 0-0. 5 mmol/L. Compared with H2O2 control group,both anthocyanins could increase the levels of T-AOC and SOD,concentration ofGSH,and decrease the concentration of MDA in HepG-2 cells at different concentration of 50,200 and 400 μmol/L. The antioxidant capacity positively correlated with the concentration of anthocyanins. The antioxidant effect of Cy was significantly higher than that of C3G. The study could provide a theoretical basis for further understanding the structure-activity relationship of anthocyanin in vivo.

关 键 词：矢车菊素 矢车菊素-3-O-葡萄糖苷 H2O2 氧化应激模型 

分 类 号：TQ028.8[化学工程]