以DNA四面体为载体研究CpG对免疫Melan-A抗原肽协同作用  

Using DNA tetrahedron as nanocarrier to study the synergistic effects of CpG on Melan-A peptide

作　　者：和晨辰 尹泽清 孙丽范 于露 洪恩律 石文琦 李海东[2] 沈万秋[1] HE Chen-chen;YIN Ze-qing;SUN Li-fan;YU Lu;HONG En-lu;SHI Wen-qi;LI Hai-dong;SHEN Wan-qiu(School of Pharmacy;School of Basic Medical Sciences;School of Public Health, Tianjin Medical University , Tianjin 300070, China)

机构地区：[1]天津医科大学药学院,天津300070 [2]天津医科大学基础医学院,天津300070 [3]天津医科大学公共卫生学院,天津300070

出　　处：《天津医科大学学报》2018年第3期185-187,共3页Journal of Tianjin Medical University

基　　金：国家自然科学基金面上项目基金资助(21373151)

摘　　要：目的:通过将Melan-A抗原肽与CpG连接在DNA四面体上,并控制肽链与CpG之间的距离和CpG的个数,研究CpG对具有免疫原性的Melan-A抗原肽的协同作用。方法:将特定设计合成好的四面体-肽、四面体-肽-CpG(I)、四面体-肽-CpG(II)注射到小鼠体内,运用ELISA法检测小鼠血清中IL-6的浓度。结果:相应产生的IL-6的浓度为9.238 pg/mL、17.150 pg/mL、18.865 pg/mL。结论:CpG增强了Melan-A抗原肽的免疫作用,并且CpG个数越多增强效果越好。Objective： To study- the synergistic efteets of CpG oligodeoxynueleotides and Melan-A antigen peptide by adding a Melan-A peptide and varied numbers of CpGs to the specified positions of a DNA tetrahedron nanostrueture. Methods： Special designed DNA tetrahedron-peptide, DNA tetrahedron-peptide-CpG（I） and DNA tetrahedron-peptide-CpG （II） nanostruetures were prepared and injected into mice. ELISA method was then used to detect the level of eytokine interleukin（IL） -6 in mice serums. Results： The concentrations of IL- 6 were 9.238 pg/mL, 17.150 pg/mL, and 18.865 pg/mL, respectively. Conclusion： CpG could enhance the immunization effect of the Melan-A antigen peptide, and the effect becomes greater with increased number of CpGs added to the DNA tetrahedron nanostrueture.

关 键 词：DNA四面体 Melan-A抗原肽 CPG 协同作用 小鼠 

分 类 号：R9[医药卫生—药学]