高流体静力压下脾静脉和大隐静脉基质金属蛋白酶及抑制物失调研究  

Imbalance in matrix metalloproteinases and matrix metalloproteinase inhibitors from diseased splenic vein and varicose great saphenous vein under high hemodynamics

作　　者：李坤[1,2] 李宏[3] 李源[3] 徐永波[2] 褚海波[2] 王豪夫[4] LI Kun;LI Hong;LI Yuan;XU Yong-bo;CHU Hai-bo;WANGHao-fu(Graduate school of Qingdao university（Qingdao 266071, China;Medical Research Center, Weifang Medical University（Weifang 261053, China;Department of General Surgery, the 89th Hospital of PLA （Weifang 261021, China;Department of Vascular Surgery, The Affiliated Hospital of Qingdao University（Qing- dao 266003, China)

机构地区：[1]青岛大学研究生院,山东青岛266071 [2]中国人民解放军第八十九医院普外中心,山东潍坊261021 [3]潍坊医学院医学研究中心,山东潍坊261053 [4]青岛大学附属医院血管外科,山东青岛266003

出　　处：《中国现代普通外科进展》2018年第2期90-94,共5页Chinese Journal of Current Advances in General Surgery

基　　金：潍坊市科技发展计划基金(2014zj1058)

摘　　要：目的:探讨高流体静力压下脾静脉和大隐静脉管壁基质金属蛋白酶及抑制物的差异。方法:收集病脾静脉(DSVs)、正常脾静脉(NSVs)、曲张大隐静脉(VGSVs)、正常大隐静脉(NGSVs)标本72份。观察4组静脉管壁MMP-2、MMP-9、TIMP-1、TIMP-2蛋白表达。检测4组静脉管壁MMP-2、MMP-9、TIMP-1、TIMP-2蛋白阳性表达比率和m RNA表达。结果:在DSVs和VGSVs管壁中,MMP-2、MMP-9、TIMP-1、TIMP-2蛋白呈簇状强阳性表达。DSVs组管壁MMP-2、MMP-9、TIMP-1、TIMP-2蛋白阳性表达比率和m RNA表达与NSVs相比显著增多,差异有统计学意义(P<0.05);VGSVs组与NGSVs组相比显著增多,差异有统计学意义(P<0.05);VGSVs/NGSVs比值与DSVs/NSVs比值相比显著增高,差异亦有统计学意义(P<0.05)。结论:高流体静力压下,脾静脉和大隐静脉管壁基质金属蛋白酶及抑制物平衡失调,这可能是静脉管壁重塑的分子机制之一。Objective： To investigate the differences in matrix metalloproteinases （MMPs） andmatrix metalloproteinase inhibitors （TIMPs） from diseased splenic vein （DSVs） and varicose greatsaphenous vein （VGSVs） under high hemodynamics. Methods： Seventy-two specimens of DSVs,normal splenic veins （SVs）, VGSVs, and normal great saphenous vein （GSVs） were collected. Venouswall in the four groups, MMP-2, MMP-9, TIMP-1, and TIMP-2 protein expression were observedand MMP-2, MMP-9, TIMP-1, TIMP-2 proteins positive expression ratio and mRNA expressionwere determined. Results： DSVs and VGSVs in the two groups, MMP-2, MMP-9, TIMP-1, TIMP-2proteins with clustered strong expression were observed; In DSVs group, MMP-2, MMP-9, TIMP-1,TIMP-2 protein positive expression ratio andmRNA expression were significantly increased comparedwith SVs group, while in VGSVs group, MMP-2, MMP-9, TIMP-1, TIMP-2 protein positive expressionratio and mRNA expression were significantly increased compared with GSVs group （ 〈0.05）.VGSVs/GSVs ratio was significantly increased compared with DSVs/SVs ratio （ 〈0.05）. Conclusion：Under high hemodynamics, the dysequilibrium of MMPs and TIMPs from splenic vein and greatsaphenous vein, These results may be one of themolecularmechanismin vascular remodeling.

关 键 词：静脉曲张 高压性脾静脉 基质金属蛋白酶 基质金属蛋白酶抑制物 

分 类 号：R654.3[医药卫生—外科学]