黄淮麦区主要推广小麦品种(系)DNA指纹图谱构建及遗传多样性分析  

Establishment of DNA Fingerprinting and Genetic Diversity Analysis of Main Wheat Cultivars in Huanghuai Region

作　　者：杨艳红 尹华燕 高雨 赵金晓 马信[1] 王宏伟[1] 李安飞[1] 孔令让[1] 李宪彬[1] YANG Yang-hong;YIN Hua-yan;GAO Yu;ZHAO JIN-xiao;MA Xin;WANGHong-wei;LI An-fei;KONG Ling-rang;LI Xian-bin(College of Agronomy/Shandong Agricultural University, Tai’an 271018, China)

机构地区：[1]山东农业大学农学院,山东泰安271018

出　　处：《山东农业大学学报（自然科学版）》2018年第3期371-378,共8页Journal of Shandong Agricultural University：Natural Science Edition

基　　金：山东省自主创新重大关键技术(2014GJJS0201-3-1);山东省农业良种工程

摘　　要：小麦作为世界上重要的粮食作物,其丰富的遗传变异对小麦的遗传改良工作至关重要。本研究利用均匀分布在小麦A、B、D基因组上的84对SSR引物对119份黄淮麦区推广小麦品种进行了DNA指纹数据库的构建,并进行遗传多样性分析。结果表明,84对SSR引物共检测到170个等位变异,每对引物检测出1~5个等位变异,其中有13对引物可以将某一小麦与其他品种区分开。根据SSR基因型聚类结果可将119份小麦品种(系)分为5大类群,基本能够分辨各个推广品种间的亲缘关系,为小麦亲本选择及杂交组配提供了一定的理论依据。Wheat is an important food crop in the world, its rich genetic variation is crucial to wheat improvement. Eighty-four SSR primers distributed on A, B, D genome were used to build DNA fingerprinting database and genetic diversityanalysis in 119 samples of main wheat cultivars in Huanghuai region. The study showed that 170 variations were detected byusing the 84 pairs of SSR primers, and each pair of primers exhibited 1-5 alleles in 119 wheat cultivars in Huanghuai region.Out of the 84 pairs of SSR primers, 13 pairs of primers can detect one of wheat cultivars different from the other varietiesrespectively. According to SSR gene clustering dendrogram, 119 wheat varieties could be divided into 5 groups to someextent, which could provide some theoretical information for wheat improvement in the future.

关 键 词：小麦 SSR引物 指纹图谱 遗传相似性 

分 类 号：S512.1[农业科学—作物学]