辣椒疫霉效应因子RxLR115890不同侵染时期的差异性表达  

Differential Expression of Phytophthora capsici Effector RxLR115890 at Different Stages of Infection

作　　者：张丽[1] 王俪颖 梁涛 丁鹏 朱彤彤 张修国[1] 李壮[1] ZHANG Li;WANG Li-ying;LIANG Tao;DING Peng;ZHU Tong-tong;ZHANG Xiu-guo;LI Zhuang(College of Plant Protection/Shandong Agricultural University, Key Laboratory of Vegetable Pest and Biology of ShandongProvince, Tai’an 271018, China)

机构地区：[1]山东农业大学植物保护学院山东省蔬菜病虫生物学省级重点实验室,山东泰安271018

出　　处：《山东农业大学学报（自然科学版）》2018年第3期388-392,共5页Journal of Shandong Agricultural University：Natural Science Edition

基　　金：国家大宗蔬菜产业技术体系(CARS-25-03B)

摘　　要：辣椒疫霉菌(Phytophthora capsici)能够产生370多个RxLR效应分子,绝大多数效应分子的特性都是未知的。为了对辣椒疫霉RxLR效应因子开展深入的功能研究,本研究以辣椒疫霉SD33菌株的DNA为模板,设计辣椒疫霉菌以及RxLR115890的特异性引物。采用实时荧光定量PCR检测RxLR115890在辣椒疫霉游动孢子侵染条件下,不同时间的表达量。结果显示,RxLR115890的表达量在侵染前期有明显的上调表达,伴随着侵染时间的加长表达量迅速下降,又在12 h处再次轻微上调。由此得出结论,RxLR115890对辣椒疫霉菌的前期侵染具有至关重要的作用。为RxLR115890后续的研奠定了基础。Phytophthora capsici can secret more than 370 RxLR effectors, and the characteristic of most effectors areunknown. In order to conduct deep functional studies on the RxLR effectors of P. capsici, the specific primers for P. capsiciand RxLR115890, were designed using the DNA of the P. capsici strain SD33 as a template. Real-time fluorescentquantitative PCR was used to detect the expression level of RxLR115890 under the zoospores infection condition of P.capsici in different stages. The results showed that the expression level of RxLR115890 was significantly up-regulated at theearly stage of infection, and the length of expression was decreased rapidly along with the infestation time, and slightlyincreased again at 12 hours. It was concluded that RxLR115890 has a crucial role in the early infection of P. capsici.Furthermore, it established the foundation for the following research of RxLR115890.

关 键 词：辣椒疫霉 RxLR115890 实时荧光定量PCR 表达量 

分 类 号：S436.418.12[农业科学—农业昆虫与害虫防治]