原花青素对猪精液常温保存效果的影响  

Effect of Procyanidins on Preservation of Porcine Sperm at Room Temperature

作　　者：胡亚美[1] 刘斌[1] 孙敬春 梁国栋 褚瑰燕[1] 杨公社[1] HU Ya-mei;LIU Bin;SUN Jing-chun;LIANG Guo-dong;CHU Gui-yan;YANG Gong-she(College of Animal Science and Technology, Northwest A＆F University, Shaanxi Yangling 712100, China;Zhongkang livestock breeding company of Dali country, Shaanxi Dali 715103, China)

机构地区：[1]西北农林科技大学动物科技学院,陕西杨凌712100 [2]大荔县众康家畜良种繁育有限公司,陕西大荔715103

出　　处：《中国畜牧杂志》2018年第5期54-57,96,共5页Chinese Journal of Animal Science

基　　金：国家生猪产业技术体系(CARS-36);陕西省重大科技创新专项资金项目(2017ZKC07-114;2017ZKC07-115)

摘　　要：为探究常温保存稀释液中添加原花青素(OPC)对猪精液保存效果的影响,本实验在经典猪精液稀释液BTS中添加0、10、20、30、40、50、60、70μg/mL OPC,检测17℃保存过程中猪精子活率、质膜和顶体完整性、总抗氧化能力(T-AOC)及精液丙二醛(MDA)含量等指标。结果表明:在经典猪精液稀释液BTS中添加OPC,随着添加浓度的增大,其对精子的保护作用呈先上升后下降的趋势,且添加浓度达50μg/mL时,效果最佳,可显著提高常温保存过程中猪精子活率、质膜和顶体完整率(P<0.05);当保存至第5天时,精子活率仍为61.63%、质膜完整率为41.88%、顶体完整率为75.88%、T-AOC浓度为1.43 U/mL、MDA含量为2.37 nmol/mL。因此,在经典猪精液稀释液BTS中添加50μg/mLOPC能够提高猪精液常温保存效果,延长精液保存时间。In order to investigate the protective effect of Procyanidins（OPC） in boar semen during 17℃. The different concentrations（0, 10, 20, 30, 40, 50, 60, 70 μg/mL） of OPC were added in BTS dilution. During the process of liquid preservation at 17℃, the sperm motility, plasma membrane integrity and acrosome integrity, total antioxidant capacity（T-AOC） and malondialdehyde（MDA） were measured after 1, 3 and 5 days of preservation. The results showed that the group concentration of 50 μg/mL OPC had the best protective effect（P0.05）. After five days preservation, the sperm motility, the plasma membrane integrity and the acrosome integrity were 61.63%, 41.88% and 75.88% respectively, concentration of T-AOC and MDA were 1.43 U/mL and 2.37 nmol/mL. In conclusion, addition of OPC to BTS improved the boar sperm quality by reducing oxidative stress during liquid preservation at 17℃ and the optimum concentration was 50 μg/mL.

关 键 词：原花青素 精液 常温保存 猪 

分 类 号：S828.3[农业科学—畜牧学]