CXCL4对人真皮微血管内皮细胞的功能及分泌血管舒缩因子的影响  

Effects of CXCL4 on human dermal microvascular endothelial cells and secretion of vasomotion factors

作　　者：姜智星 杨森 陈琛 梁敏锐 邹和建 JIANG Zhi-Xing;YANG Sen;CHEN Chen;LIANG Min-Rui;ZOU He-Jian(Division of Rheumatology, Huashan Hospital, Fudan University, Shanghai 200000, Chin)

机构地区：[1]复旦大学附属华山医院风湿科,上海200000

出　　处：《中国免疫学杂志》2018年第5期665-669,共5页Chinese Journal of Immunology

基　　金：国家自然青年科学基金项目(81501391)

摘　　要：目的:探讨CXCL4对人真皮微血管内皮细胞(HDMEC)增殖能力、血管形成能力及相关因子表达的影响。方法:采用梯度浓度CXCL4干预HDMEC,通过CCK-8检测细胞增殖能力,血管形成实验观察CXCL4对HDMEC血管形成能力的影响,通过RT-PCR检测内皮素-1、Fli-1、AT1R、ETAR的mRNA水平。并使用CXCL4拮抗剂观察能否抑制CXCL4对HDMEC上述功能的影响。结果:HDMEC上可表达CXCL4特异性受体CXCR3。CXCL4可抑制HDMEC增殖及血管形成数目,且呈剂量依赖关系。CXCL4显著上调HDEMC中内皮素-1、AT1R基因的mRNA水平(P<0.05),下调Fli-1基因的mRNA水平(P<0.05),对ETAR的mRNA水平无影响。且CXCL4拮抗剂可逆转CXCL4对HDMEC的上述影响。结论:CXCL4能抑制HDMEC的增殖及血管形成,提高ET-1、AT1R的mRNA水平,降低Fli-1的mRNA水平,且呈浓度依赖性。Objective： To identify the proliferation effect and angiogenic ability of CXCL4 on human dermal microvascular endothelial cells（ HDMECs）,and to explore the secretion of vasomotion factors. Methods： HDMECs were treated with gradient concentration to test the proliferation of HDMECs. CCK-8 was used to explicated the proliferation of HDMECs. The effect of CXCL4 on angiogenic ability of HDMECs was determined by tube formation assay. The mRNA levels of endothelin-1（ ET-1）,Fli-1,Angiotensin Ⅱ type 1 receptor（ AT1R） and endothelin-1 type A receptor（ ETAR） were detected by real-time quantitative polymerase chain reaction（ Real-time PCR）. Results： The specific receptor of CXCL4 was expressed on HDMECs. CXCL4 could inhibit the proliferation of HDMECs and the number of tube formation in a dose-depend manner. After CXCL4 intervention,the relative amplification multiples of ET-1,AT1R were significantly increased（ P〈 0. 05）,Fli-1 was decreased（ P 0. 05）,and ETAR had no change as compared with the control group. Furthermore,CXCL4 antagonist could reverse the effects of CXCL4 on HDMECs. Conclusion： CXCL4 inhibit the proliferation and angiogenesis of HDMECs and induce the secretion of ET-1 and AT1R,reduce the secretion of Fli-1 in a dose-dependent manner.

关 键 词：CXCL4 系统性硬化症 内皮素-1 FLI-1 AT1R 

分 类 号：R593.25[医药卫生—内科学]