PTEN在动脉粥样硬化中的表达及对血管内皮细胞增殖凋亡的影响研究  

Expression of PTEN in atherosclerosis and its effect on proliferation and apoptosis of vascular endothelial cells

作　　者：闫博阳 赵津璋 陈虹[1] YAN Bo-Yang;ZHAO Jin-Zhang;CHEN Hong(Jiamusi City Center Hospital, Heilongjiang Province, Jiamusi 154002, China)

机构地区：[1]黑龙江省佳木斯市中心医院,佳木斯154002

出　　处：《中国免疫学杂志》2018年第5期745-750,共6页Chinese Journal of Immunology

摘　　要：目的:探讨第10号染色体同源缺失性磷酸酶-张力蛋白(PTEN)在动脉粥样硬化中的表达及对血管内皮细胞(VEC)增殖凋亡的影响。方法:构建动脉粥样硬化小鼠模型,Real-time PCR和Western blot检测动脉粥样硬化组织中PTEN水平;用不同浓度的氧化低密度脂蛋白(ox-LDL)处理大鼠VEC,噻唑蓝(MTT)检测细胞增殖活性。VEC转染PTEN过表达载体(pSicoR-PTEN),用ox-LDL处理细胞,Real-time PCR和Western blot检测PTEN水平,MTT检测细胞增殖,流式细胞术检测细胞凋亡,Western blot检测磷酸化的STAT3(p-STAT3)、信号转导与转录因子3(STAT3)、活化的含半胱氨酸的天冬氨酸蛋白水解酶3(Cleaved Caspase-3)水平。结果:动脉粥样硬化组织中的PTEN水平明显低于正常的动脉组织(P<0.05)。不同浓度的oxLDL处理后的VEC存活率明显降低,并且细胞存活率随着ox-LDL浓度的升高而降低。ox-LDL作用后的VEC中PTEN表达水平下降,而转染pSicoR-PTEN后可以提高VEC中PTEN表达水平。ox-LDL处理后的VEC凋亡率增加,细胞中Cleaved Caspase-3水平升高,p-STAT3水平下降,与正常培养的VEC相比差异具有统计学意义(P<0.05)。转染pSicoR-PTEN后VEC经过ox-LDL处理后细胞存活率、p-STAT3水平明显升高,而细胞凋亡率、Cleaved Caspase-3水平明显降低,与单纯ox-LDL处理后的细胞相比差异具有统计学意义(P<0.05)。结论:PTEN在动脉粥样硬化组织中表达下调,PTEN可以逆转ox-LDL对VEC增殖抑制和凋亡促进作用,作用机制可能与STAT3信号通路的激活有关。Objective： To investigate the expression of PTEN in atherosclerosis and its effect on the proliferation and apoptosis of vascular endothelial cell（ VEC）. Methods： The atherosclerotic mouse models were constructed,and the levels of PTEN in atherosclerotic tissues were detected by Real-time,PCR and Western blot; Rat VEC was treated with different concentrations of ox-LDL,cell proliferation activity was detected by MTT. VEC was transfected with PTEN overexpression vector（ pSicoR-PTEN）,processing cells with ox-LDL,the levels of PTEN was detected by Real-time,PCR and Western blot,cell proliferation was detected by MTT,apoptosis was detected by flow cytometry,the levels of p-STAT3,STAT3 and Cleaved Caspase-3 were detected by Western blot. Results： The level of PTEN in atherosclerotic tissue was lower than that in normal arterial tissue（ P〈0. 05）. The survival rate of VEC decreased obviously after treatment with different concentrations of ox-LDL,and the cell survival rate decreased with the increase of ox-LDL concentration. The expression of PTEN decreased in VEC after ox-LDL action,transfection of pSicoR-PTEN could increase the expression level of PTEN in VEC. The apoptotic rate of VEC increased after ox-LDL treatment,elevated levels of Cleaved Caspase-3 in cells,the level of p-STAT3 decreased,compared with normal VEC,and the difference was statistically significant（ P 0. 05）. After transfection of p SicoR-PTEN,the survival rate and p-STAT3 level of VEC were significantly increased after ox-LDL treatment,but the apoptosis rate and the level of Cleaved Caspase-3 were significantly decreased,compared with the cells treated with pure ox-LDL,the difference was statistically significant（ P〈0. 05）. Conclusion： Downregulation of PTEN expression in atherosclerosis,PTEN inhibits the proliferation and apoptosis of VEC by ox-LDL,the mechanism of action may be related to the activation of STAT3 signaling pathway.

关 键 词：动脉粥样硬化 血管内皮细胞 PTEN 凋亡 

分 类 号：R543.5[医药卫生—心血管疾病]