慢性萎缩性胃炎患者Hp感染与TGF-βRⅡ、IL-6和TNF-α的表达研究  

Hp infection and expression of TGF-βR Ⅱ,IL-6 and TNF-α in patients with chronic atrophic gastritis

作　　者：黄懋敏 董丹丹[2] 亓丹丹 凌晨 张丁丁 HUANG Mao-Min;DONG Dan-Dan;QI Dan-Dan;LING Chen;ZHANG Ding-Ding(Department of Immunology, Zunyi Medical University, Zunyi 563000, Chin)

机构地区：[1]遵义医学院免疫教研室,遵义563000 [2]四川省医学科学院.四川省人民医院病理科,成都610072 [3]四川省医学科学院.四川省人民医院人类疾病基因研究重点实验室,成都610072 [4]电子科技大学医学院,成都611731

出　　处：《中国免疫学杂志》2018年第5期751-756,共6页Chinese Journal of Immunology

摘　　要：目的:探讨慢性萎缩性胃炎患者幽门螺旋杆菌感染与胃黏膜中转化生长因子β受体Ⅱ、白介素6和肿瘤坏死因子α的表达情况。方法:选取76例慢性萎缩性胃炎(CAG)患者胃黏膜病变组织的胃镜活检标本,采取PCR荧光法检测Hp感染情况,免疫组化法检测TGF-βRⅡ、IL-6和TNF-α在胃小凹上皮和间质炎细胞中的表达。结果:慢性炎症程度在Hp感染阳性组和阴性组中差异有统计学意义(P<0.05);间质炎细胞中IL-6的表达在Hp感染阳性组和阴性组中差异有统计学意义(P<0.05);TGF-βRⅡ、TNF-α的表达在Hp感染阳性组和阴性组中无显著性差异(P>0.05);间质炎细胞中IL-6与慢性炎症程度呈正相关关系(r=0.249,P=0.03);萎缩程度与肠上皮化生严重程度、上皮内瘤变程度呈正相关关系(r=0.697,0.366)。结论:IL-6在间质炎性细胞中的表达与Hp相关性CAG患者的慢性炎症程度有关,慢性萎缩程度促进肠上皮化生和上皮内瘤变的发生。Objective： To investigate the effect of Helicobacter pylori（ Hp） infection in patients with chronic atrophic gastritis（ CAG） and the expression of transforming growth factor-β receptor Ⅱ,interleukin 6 and tumor necrosis factor-α in gastric mucosa. Methods： 76 cases of gastroscope biopsy specimens were collected in the CAG patients,the infection of Hp was detected by PCR fluorescence,and immunohistochemical staining was used to determine the expression of TGF-βRⅡ,IL-6 and TNF-α in gastric foveolar epithelium and stromal inflammatory cells. Results： There were significant differences in chronic inflammation between Hp-positive and Hp-negative group（ P〈0. 05）. Expression of IL-6 in stromal inflammatory cells was significantly different between Hp-positive and Hp-negative group（ P〈0. 05）. Expression of TGF-βRⅡ and TNF-α was not significantly different in two groups（ P〉0. 05）. IL-6 expressed instromal inflammatory cells were correlated with chronic inflammation（ r = 0. 249,P = 0. 03）. The degree of intestinal metaplasia and dysplasia were correlated with the atrophic severity respectively（ r = 0. 697,0. 366）. Conclusion： IL-6 is related to the chronic inflammation in patients with CAG who has Hp infection. The chronic atroplic severity promotes the development of intestinal metaplasia and dysplasia.

关 键 词：幽门螺旋杆菌 转化生长因子-β受体Ⅱ 白介素-6 肿瘤坏死因子-α PCR荧光法 免疫组化 

分 类 号：R392[医药卫生—免疫学]