Elucidating the various multi-phosphorylation statuses of protein functional regions by 193-nm ultraviolet photodissociation  

作　　者：Zheyi Liu You Jiang Chunlei Xiao Xingchuang Xiong Tao Wang Jinan Li Fangjun Wang Xiang Fang Xueming Yang 

机构地区：[1]CAS Key Laboratory of Separation Sciences for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China [2]National Institute of Metrology, Beijing 100012, China [3]State Key Laboratory of Molecular Reaction Dynamics, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China

出　　处：《Chinese Chemical Letters》2018年第5期694-698,共5页中国化学快报（英文版）

基　　金：Financial supports are gratefully acknowledged for the China State Key Research Grant(No.2016YFF0200504);China State Key Basic Research Program Grant(No.2013CB911203);the National Natural Science Foundation of China(No.21675152);the Youth Innovation Promotion Association CAS(No.2014164);grant from DICP(No.ZZBS201603)

摘　　要：Ultraviolet photodissociation is a high-energy fast excitation method in mass spectrometry and has beensuccessfully applied for the elucidation of sequences and structures of biomolecules. However, its abilityto distinguish the phosphorylation sites isomers of multi-phosphopeptides has been not systematicallyinvestigated until now. A 193-nm ultraviolet laser dissociation mass spectrometry system wasestablished in this study and applied to elucidate the complex multi-phosphorylation statuses mimickingthe functional regions of Sicl, Gli3 and Tau. The numbers of matched fragment ions and phosphorylationsite-determining ions were improved on average 123% and 104%, respectively, by utilizing the ultravioletphotodissociation strategy, comparing to the typically utilized collision induced dissociation strategy.Finally. 94% phosphorylation sites within various statuses were unambiguously elucidated.Ultraviolet photodissociation is a high-energy fast excitation method in mass spectrometry and has beensuccessfully applied for the elucidation of sequences and structures of biomolecules. However, its abilityto distinguish the phosphorylation sites isomers of multi-phosphopeptides has been not systematicallyinvestigated until now. A 193-nm ultraviolet laser dissociation mass spectrometry system wasestablished in this study and applied to elucidate the complex multi-phosphorylation statuses mimickingthe functional regions of Sicl, Gli3 and Tau. The numbers of matched fragment ions and phosphorylationsite-determining ions were improved on average 123% and 104%, respectively, by utilizing the ultravioletphotodissociation strategy, comparing to the typically utilized collision induced dissociation strategy.Finally. 94% phosphorylation sites within various statuses were unambiguously elucidated.

关 键 词：Ultraviolet photodissociation Mass spectrometry Multi-phosphorylation Phosphorylated tyrosine Phosphorylation site-determining ion 

分 类 号：O629.73[理学—有机化学]