茵栀黄注射液对胆汁淤积性肝炎大鼠氧化应激反应及FXR基因表达的影响  

Effects of Yinzhihuang Injection on Oxidative Stress and FXR Gene Expression in Cholestatic Hepatitis Rats

作　　者：谭梅傲 康锦花 佘世锋[2] 曹敏[1] 李琦[1] 吴海滨[3] 梁美均 TAN Mei-Ao;KANG Jin-Hua;SHE Shi-Feng;CAO Min;LI Qi;WU Hai-Bin;LIANG Mei-Jun(Guangzhou University of Chinese Medicine, Guangzhou 510405 Guangdong, China;Dept. of Gastroenterology, the First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou 510405 Guangdong, China;Shenzhen Hospital of Traditional Chinese Medicine, Shenzhen 518033 Guangdong, China)

机构地区：[1]广州中医药大学,广东广州510405 [2]广州中医药大学第一附属医院脾胃病科,广东广州510405 [3]深圳市中医院,广东深圳518033

出　　处：《广州中医药大学学报》2018年第3期466-470,共5页Journal of Guangzhou University of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(编号:81173437)

摘　　要：【目的】探讨茵栀黄注射液对胆汁淤积性肝炎的改善作用及可能的机制。【方法】采用α-异硫氰酸萘酯(ANIT)灌胃法复制胆汁淤积性肝炎大鼠模型。将28只雄性Wistar大鼠随机分为正常对照组、模型组、茵栀黄注射液组(YI组)、熊去氧胆酸组(UDCA组),每组7只。造模结束后,采用全自动生化仪检测各组大鼠血清谷丙转氨酶(ALT)、谷草转氨酶(AST)、碱性磷酸酶(ALP)、谷氨酰转肽酶(GGT)、总胆红素(TB)水平,采用比色法检测血清超氧化物歧化酶(SOD)、丙二醛(MDA)活性变化,采用苏木素—伊红(HE)染色法观察肝组织病理改变,采用反转录实时荧光定量聚合酶链反应(RT-q PCR)法检测肝组织法尼酯X受体(FXR)m RNA的表达。【结果】与正常对照组比较,模型组大鼠出现明显的肝脏病理损害,血清ALT、AST、ALP、TB水平,肝组织MDA活性、FXR m RNA表达水平均显著升高,肝组织SOD活性下降(P<0.05)。与模型组比较,YI组大鼠肝脏病理损害明显减轻,血清ALT、AST、ALP、TB水平及肝组织MDA活性均显著下降,FXR m RNA表达水平及肝组织SOD活性上升(P<0.05)。【结论】茵栀黄注射液能显著改善肝内胆汁淤积性肝炎大鼠肝损伤,其机制可能与抑制氧化应激反应、促进FXR基因表达有关。Objective To investigate the protective effect of Yinzhihuang Injection（YI）against cholestatic liverdisease and to explore its possible mechanism. Methods Twenty-eight male Wistar rats were randomly divided intonormal control group,model group,YI group,ursodeoxycholic acid（UDCA）group,7 rats in each group. Ratmodel of cholestatic hepatitis was established by intra-gastric administration of alpha-naphthyl isothiocyanate（ANIT）. The serum levels of alanine aminotransferase（ALT）, aspartate aminotransferase（AST）, alkalinephosphatase（ALP）,gamma-glutamyl transpeptidase（GGT）,and total bilirubin（TB）were tested by automaticbiochemical analyzer,the activity of superoxide dismutase（SOD）and malondialdehyde（MDA）was determined bycolorimetric method, the pathological changes of hepatic tissues were observed by hematoxylin-eosin（HE）staining, and the expression level of farnesoid X receptor（FXR）m RNA was detected by real time reverse transcription quantitative polymerase chain reaction（RT-q PCR）. Results Compared with the normal blank group,the damage in rat hepatic tissues of the model group was obvious,the serum ALT,AST,ALP,TB levels and thehepatic MDA activity and FXR m RNA expression level were increased,and hepatic SOD activity was decreased（P〈0.05）. Compared with the model group,the damage in rat hepatic tissues of YI group was relieved,theserum ALT, AST, ALP, TB levels, the hepatic MDA activity were decreased, and FXR m RNA expressionlevel and hepatic SOD activity was increased（P〈0.05）. Conclusion YI can significantly improve liver injury incholestatic hepatitis rats, and its possible mechanism may be related with the inhibition of oxidative stressresponse and the enhancement of FXR gene expression.

关 键 词：茵栀黄注射液 胆汁淤积性肝炎 氧化应激 法尼酯X受体 基因表达调控 疾病模型 动物 大鼠 

分 类 号：R285.5[医药卫生—中药学]