Metabolite characterization of Penta-O-galloyl-β-D-glucose in rat biofluids by HPLC-QTOF-MS  

作　　者：cui-xia ma xue zhao pei wang pu jia xin-feng zhao chao-ni xiao xiao-hui zheng Cui-xia Ma, Xue Zhao, Pei Wang, Pu Jia, xin-feng Zhao, Chao-ni Xiao, Xiao-hui Zheng(Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, College of Life Sciences, Northwest University, Xi＇an 710069, Chin)

机构地区：[1]key laboratory of resource biology and biotechnology in western china,ministry of education,college of life sciences,northwest university,Xi'an 710069 China

出　　处：《Chinese Herbal Medicines》2018年第1期73-79,共7页中草药（英文版）

基　　金：the National Key Scientific Instrument and Development Project of China (2013YQ170525);the Program for Changjiang Scholars and Innovative Research Team in University (no.IRT15R55);National Natural Science Foundation of China (no.81603259);Natural Science Foundation of Shaanxi Province (2015JM2072)

摘　　要：Objective： To understand the in vivo metabolic fate of 1,2,3,4,6-Penta-O-galloyl-β-d-glucose（PGG）naturally existed in many medicinal herbs and food plants such as Rhus chinensis,Paeonia suffruticosa,Paeonia lactiflora and Mango.Methods： The metabolites of PGG in rat biofluids were characterized using high performance liquid chromatography combined with quadrupole time-of-flight tandem mass spectrometry（HPLC-QTOF-MS）.Results： Ten metabolites in urine,five metabolites in feces and two metabolites in plasma,were observed when the rats were administrated with a single intravenous injection of PGG（20 mg/kg）.Conclusion： PGG is firstly metabolized to gallic acid,then gallic acid undergoes sulfation,glucuronidation and methylation by rat liver.The determination of metabolites and the proposed metabolic pathway of PGG in vivo will be benefit to gain deeper insights into its pharmacological activities.Objective： To understand the in vivo metabolic fate of 1,2,3,4,6-Penta-O-galloyl-β-d-glucose（PGG）naturally existed in many medicinal herbs and food plants such as Rhus chinensis,Paeonia suffruticosa,Paeonia lactiflora and Mango.Methods： The metabolites of PGG in rat biofluids were characterized using high performance liquid chromatography combined with quadrupole time-of-flight tandem mass spectrometry（HPLC-QTOF-MS）.Results： Ten metabolites in urine,five metabolites in feces and two metabolites in plasma,were observed when the rats were administrated with a single intravenous injection of PGG（20 mg/kg）.Conclusion： PGG is firstly metabolized to gallic acid,then gallic acid undergoes sulfation,glucuronidation and methylation by rat liver.The determination of metabolites and the proposed metabolic pathway of PGG in vivo will be benefit to gain deeper insights into its pharmacological activities.

关 键 词：1 2 3 4 6-Penta-O-galloyl-β-D-glucose （PGG）HPLC-QTOF-MSmetabolic pathway 

分 类 号：R285.5[医药卫生—中药学]