黄花苜蓿花药培养再生体系的建立与单倍体鉴定  

Establishment of a regeneration system with anther tissue culture of Medicago falcata 'Hulunbeier' and haploid identification

作　　者：徐舶 高霞 石凤翎 崔楠 乌日娜 Xu Bo;Gao Xia;Shi Feng-ling;Cui Nan;Wurina(College of grassland,resources and environment, Inner Mongolia Agricultural University, Hohhot 010019, Inner Mongolia, China)

机构地区：[1]内蒙古农业大学草原与资源环境学院,内蒙古呼和浩特010019

出　　处：《草业科学》2018年第5期1090-1097,共8页Pratacultural Science

基　　金：国家自然科学基金项目(31402126);国家自然科学基金项目(31260576);内蒙古自治区草原英才"内蒙古高原乡土草种质资源遗传改良与利用"创新团队;草地资源教育部重点实验室

摘　　要：花药组织培养再生体系的构建是单倍体育种的重要途径之一。本研究对呼伦贝尔黄花苜蓿(Medicago falcate‘Hulunbeier’)进行了花药组织培养研究并建立了花药组培再生体系。结果显示,液体悬浮培养基比固体培养基更适合呼伦贝尔黄花苜蓿花药愈伤组织的培养,其愈伤形成的培养条件为B5培养基+0.5 mg·L^(-1) 2,4-D+0.25 mg·L^(-1) 6-BA+0.4mg·L^(-1) NAA+3.0mg·L^(-1) KT;分化培养基为MS+1.0mg·L^(-1) 2,4-D+0.5mg·L^(-1) 6-BA+2%蔗糖+0.7%琼脂;生根培养基为1/2MS+0.1mg·L^(-1) NAA+2%蔗糖+0.7%琼脂;获得的再生植株经流式细胞仪鉴定,其单倍体比例高达27%。The establishment of a regeneration system using the anther tissue culture method is an important approach in haploid breeding.In the present study,the anther tissue culture of Medicago falcata ‘Hulunbeier＇was investigated and the regeneration system of anther tissue culture established.The results showed that liquid suspension medium was more suitable than solid medium for M.falcatain the induction of anther callus.Mixtures of B5＋0.5 mg·L^（-1） 2,4-D＋0.25 mg·L^（-1） 6-BA＋0.4 mg·L^（-1） NAA＋3.0 mg·L^（-1） KT,MS＋1 mg·L^（-1） 2,4-D＋0.5 mg·L^（-1） 6-BA＋sucrose 2%＋ager 0.7%,and 1/2 MS＋0.1 mg·L^（-1） NAA＋sucrose2%＋ager 0.7% were suitable for callus induction,callus differentiation,and rooting culture,respectively.The percentage of haploids of regenerated plants was measured by flow cytometry as being 27%for M.falcata.

关 键 词：花药组织培养 黄花苜蓿 倍性鉴定 单倍体 液体悬浮培养 激素 流式细胞术 

分 类 号：S551.704.8[农业科学—作物学] Q945.39[生物学—植物学]