黄芪和当归配伍对大鼠血管内膜增生血管平滑肌细胞增殖的影响  

Effects of Astragalus-Angelica combination on VSMC proliferation of vascular intimal hyperplasia in rats

作　　者：阎卉芳[1] 徐昊[1] 彭熙炜 朱嘉欢 邓常清[1] YAN Huifang;XU Hao;PENG Xiwei;ZHU Jiahuan;DENG Changqing(Key Laboratory of Hunan Province for Integrated Traditional Chinese and Western Medicine on Prevention and Treatment of Cardio-Cerebral Disease, Hunan University of Chinese Medicine, Changsha 410208, Hunan, Chi-na;Department of Cardiology, Affiliated Changde Hospital of Hunan University of Chinese Medicine, Changde 415000 ,Hunan , China)

机构地区：[1]湖南中医药大学中西医结合心脑疾病防治湖南省重点实验室 [2]湖南中医药大学附属常德医院心血管科

出　　处：《中国临床药理学与治疗学》2018年第4期361-369,共9页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：国家自然科学基金面上项目(81473581);"中西医结合防治心脑血管疾病的相关基础研究"湖南省科技创新团队(2013年);"中医药防治心脑血管疾病基础研究"湖南省自然科学创新群体基金(2013年)

摘　　要：目的:探讨黄芪和当归不同配伍对大鼠血管内皮损伤后血管内膜增生中血管平滑肌细胞(VSMC)表型转化、细胞周期和磷脂酰肌醇3-激酶(PI3K)/蛋白激酶B(Akt)信号途径的影响。方法:雄性大鼠制备腹主动脉球囊导管损伤血管内皮模型,设计黄芪和当归单用及不同比例配伍,对内皮损伤模型大鼠灌胃给药,干预14 d后取腹主动脉,检测腹主动脉增生内膜中VSMC表型转化、细胞周期相关蛋白表达和PI3K/Akt信号通路相关蛋白的表达。结果:球囊导管损伤主动脉内皮后,可引起VSMC表型转化及VSMC增殖从而导致血管内膜增生。当归、黄芪、黄芪-当归1∶1配伍、黄芪-当归5∶1配伍可增强血管增生内膜中平滑肌α肌动蛋白(SMα-actin)表达,抑制增生内膜中增殖细胞核抗原(PCNA)蛋白表达。当归、黄芪、黄芪-当归1∶1配伍、黄芪-当归5∶1配伍可抑制血管组织中cyclin D1、cyclin E表达的增高,抑制增生内膜中p-PI3K和p-Akt蛋白表达的升高。结论:黄芪当归配伍对血管内皮受损后内膜增生具有抑制作用,其作用可能与其通过抑制血管内皮受损后PI3K/Akt信号通路激活,进而抑制VSMC表型转化和细胞增殖,从而发挥抗VSMC增殖的作用有关。AIM： To investigate the effects of different Astragalus-Angelica combination on vascu-lar smooth muscle cells （VSMC） phenotype transformation, cell cycle and PI3K/AKT signal pathway of vascular intimal hyperplasia in rats. METHODS：Amodel of intimal hyperplasia of thoracoabdominal aorta was established by balloon catheter injury in male Sprague-Dawley rat, and the model rats were administrated Astragalus and Angelica with single use or different ratios for 14 days by means of intra-gastric administration.Then the thoracoabdominal aorta was taken out to analyze the pathological chan-ges of the VSMC, expression of cell cycle and PI3K/Akt signaling pathway related proteins in hyperplastic intima.RESULTS： After balloon injury of aortic endothelium, VSMC phenotype transformation and proliferation can be induced,resulting in intimal hyperplasia.In hyperplastic intima,the expression of SM-actin can be enhanced and the expression of PCNA can be inhibited by the Astragalus and Angelica with single use and Astragalus-Angelica of 1：1 and 5：1 combination.The increased expression of cyclinD1 or cyclinE in damaged blood vessels and p-PI3K or p-Akt protein in hyperplastic intima can be inhibited by the Astragalus and Angelica with single use and Astragalus-Angelica of 1：1 and 5：1 combination.CONCLUSION：Vascular intimal hyperplasia can be inhibited by Astragalus-Angelica compatilibity.The mechanism may be related to the inhibi-tion of PI3K/Akt signaling pathway activation,which lead to the inhibition of the phenotypie trans-formation and cell proliferation of VSMC,and then play the role of anti VSMC proliferation.

关 键 词：黄芪 当归 血管平滑肌细胞 细胞周期 磷脂酰肌醇3-激酶/蛋白激酶B(PI3K/Akt) 

分 类 号：R965.2[医药卫生—药理学]