亚硝酸盐胁迫下异育银鲫呼吸代谢生理响应  

Respiratory metabolic responses of Carassius auratus gibelio to nitrite stress

作　　者：张晓莹 么宗利[1] 来琦芳[1] 厉成新 周凯[1] 刘进红 隋延鸣[1] 马庆男 高鹏程 ZHANG Xiao-ying 1,2 , YAO Zong-li1, LAI Qi-fang1, LI Cheng-xin3 ,ZHOU Kai1, LIU Jin-hong3 , SUI Yan-ming1, MA Qing-nan3 , GAO Peng-cheng1(1. East China Sea Fisheries Research Institute, Engineering Research Center for Saline-alkaline Water Fisheries, Chinese Academy of Fishery Sciences, Shanghai 200090, China;2. College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China; 3. Bright Fisheries Company Limited, Dafeng Jiangsu 224153, Chin)

机构地区：[1]中国水产科学研究院东海水产研究所盐碱水域渔业工程技术研究中心,上海200090 [2]上海海洋大学水产与生命学院,上海201306 [3]光明渔业有限公司,江苏大丰224153

出　　处：《海洋渔业》2018年第2期189-196,共8页Marine Fisheries

基　　金：上海市科技兴农重点攻关项目[沪农科攻字(2015)第1-6号];上海市科学技术委员会科研计划项目(14ZR1449800)

摘　　要：高浓度亚硝酸盐会影响鱼类的健康甚至生存,为深入了解亚硝酸盐胁迫下异育银鲫(Carassius auratus gibelio)呼吸代谢生理响应,采用急性胁迫方法,将异育银鲫幼鱼置于亚硝酸盐浓度为0.5、1、1.5、2、3mg·L^(-1)的水环境中胁迫96 h并监测标准代谢率(SMR)、最大代谢率(MMR)、有氧代谢范围(AS)及高铁血红蛋白比例(MHb/Hb)的变化规律。结果显示:随水环境亚硝酸盐浓度的升高,异育银鲫SMR显著升高(P<0.05),MMR显著降低(P<0.05),AS显著降低(P<0.05),MHb/Hb显著升高(P<0.05)。异育银鲫的SMR、MMR、AS与水环境亚硝酸盐浓度均呈二次方回归关系,血液中MHb/Hb与水环境亚硝酸盐浓度呈三次方回归关系。结果表明:异育银鲫SMR与水环境亚硝酸盐浓度呈正相关,MMR及AS与之呈负相关。在高浓度亚硝酸盐水环境中,鱼体升高SMR用于修复自身机能并维持基本生理功能;MHb/Hb显著升高,导致载氧能力下降,可供给鱼体的氧气量减少,MMR显著降低,游泳速度下降,捕食及逃逸等活动受到限制;AS显著降低,不能满足鱼类摄食生长等能量需求,易造成能量需求冲突,不利于鱼类的健康生长。With the improvement of people＇s living standards and the growing awareness of healthy spending,traditional fisheries have been replaced by healthy farming gradually, which requires suitable aquatic environment in farming pond. However,due to high stocking densities and improper water treatment,the changes of water quality indexes may affect the growth and quality of farmed fish. Therefore,the regulation of water quality should be enhanced. To provide basic data for the physiology and metabolism of C. auratus gibelio,the respiratory metabolic and proportion of methemoglobin（ MHb/Hb） response of C. auratus gibelio to high nitrite stress was evaluated by the method of acute exposure. C. auratus gibelio individuals were exposed to different concentrations of nitrite 0. 5,1,1. 5,2,3 mg·L^（-1）. The results showed that standard metabolic rate of C. auratus gibelio increased significantly with increasing ambient nitrite（ P〈 0. 05）. There was quadratic regression relationship between standard metabolic rate and ambient nitrite. Under 1. 5 mg ·L-1 nitrite environment,standard metabolic rate increased by 40% comparing with the control. The maximum metabolic rate decreased significantly with increasing ambient nitrite（ P〈 0. 05）. There was quadratic regression relationship between the maximum metabolic rate and ambient nitrite. Under 1. 5 mg·L-1 nitrite environment,the maximum metabolic rate decreased by 18% comparing with the control. Aerobic scope decreased significantly with increasing ambient nitrite（ P〈 0. 05）. There was quadratic regression relationship between aerobic scope and ambient nitrite. Under 1. 5 mg · L-1 nitrite environment,aerobic scope decreased by 45% comparing with the control. Proportion of methemoglobin（ MHb/Hb） increased significantly with increasing ambient nitrite（ P 〈0. 05）. There was cubic regression relationship between the concentration of proportion of methemoglobin（ MHb/Hb） and nitrite in water. Under 3 mg · L-1 nitrite environment,the proportion

关 键 词：异育银鲫 亚硝酸盐 呼吸代谢 高铁血红蛋白比例 

分 类 号：S965.1[农业科学—水产养殖]