爱尔眼库角膜供体血清学检测结果分析  

Analysis of the serological testing results from Aier Eye Bank

作　　者：龙文 杨丽红 徐婕 何圣玉 李绍伟 王丛香 王科华 冉茹秋 唐建[2] Long Wenxiu;Yang Lihong;Xu Jie;He Shengyu;Li Shaowei;Wang Congxiang;Wang Kehua;Ran Ruqiu;Tang Jian(Aier School of Ophthalmology,Central South University,Changsha 410015,China;Changsha Aier Eye Hospital,Changsha 410015,Chin;Chengdu Cambridge Eye Hospital, Chengdu 610041,Chin;Beijing Aier Intech Eye Hospital,Beijing 100021 ,Chin)

机构地区：[1]中南大学爱尔眼科学院,长沙410015 [2]成都康桥爱尔眼科医院,610041 [3]北京爱尔英智眼科医院,100021

出　　处：《中华实验眼科杂志》2018年第5期351-354,共4页Chinese Journal Of Experimental Ophthalmology

基　　金：湖南省自然科学基金项目（2017X2001）;爱尔眼科医院集团科研基金项目（AM164D05）

摘　　要：目的对长沙爱尔眼库和成都康桥爱尔眼库共640例角膜供体血清学检测结果进行分析，评价角膜供体的安全性。方法对2011年1月至2015年12月长沙爱尔眼库和成都康桥爱尔眼库角膜捐献者的血清学检测结果进行回顾性分析，采用胶体金法或酶联免疫吸附测定法（ELISA）检测乙型肝炎病毒表面抗原（HBsAg）、丙型肝炎病毒（HCV）、梅毒螺旋体（TP）和人免疫缺陷病毒（HIV）。结果640例角膜捐献者中有83例传统病血清学检测结果阳性，阳性率为12.97%。其中，60例角膜供体HBsAg阳性（占9.38%），3例角膜供体HCV抗体阳性（占0.47%），11例角膜供体TP抗体阳性（占1.72%），2例角膜供体HIV抗体阳性（占0.31%），3例角膜供体HBsAg和HCV抗体同时阳性，2例HCV抗体和TP抗体同时阳性，1例角膜供体HBsAg和HIV抗体同时阳性，1例角膜供体HBsAg和TP抗体同时阳性。结论血液传染病检测结果阳性比例较高，对角膜受体以及眼库工作者都是一个潜在的威胁。因此，对角膜供体进行严格的血清学检测非常重要。Objective This study was to evaluate the safety of 640 corneal donors by analysing the serological testing results.Methods We retrospectively analyzed the serological testing results from Changsha Aier Eye Bank and Chengdu Kangqiao Aier Eye Bank from January 2011 to December 2015, hepatitis B virus surface antigen （HBsAg）, hepatitis C virus （HCV）, treponema pallidum （TP） and human immunodeficiency virus （HIV） were detected by colloidal gold or enzyme-linked immunosorbent assay （ELISA）.Results There were 83 out of 640 serum samples showed positive immuno-reaction assayed markers, the positive rate was 12.97%, including HBsAg（n=60, 9.38%）, HCV（n=3, 0.47%）, TP（n=11, 1.72%） and HIV（n=2, 0.31%）. Moreover, 3 corneal donors were both positive against HBsAg and HCV, 2 donors positive against HCV and TP, 1 donor positive against HBsAg and HIV, 1 donor positive against HBsAg and TP.ConclusionsThere is a high proportion of positive results of blood-borne diseases in cornea donors, which is a potential threat to corneal receptors and eye bank workers.Therefore, it is very important to detect serological test strictly for corneal donors.

关 键 词：角膜供体 血清学检测 乙型肝炎表面抗原 安全性 

分 类 号：R193.3[医药卫生—卫生事业管理]