MIF基因启动子区-173多态性与冠心病相关性研究  

Macrophage Migration Inhibitory Factor Promoter Polymorphisms (-173G/C) : Relationship with Coronary Atherosclerotic Disease Subject

作　　者：钱鲁 殷日鹏[1] Qian Lu;Yin Ripeng(Department of Cardiology, The Second Affiliated Hospital, Wenzhou Medical University, Zhe jiang 325000,Chin)

机构地区：[1]温州医科大学附属第二医院,325000

出　　处：《医学研究杂志》2018年第4期32-35,共4页Journal of Medical Research

基　　金：国家自然科学基金资助项目(81573185);浙江省自然科学基金资助项目(2014C33163);温州市科技局基金资助项目(Y20130167)

摘　　要：目的 探讨巨噬细胞移动抑制因子(MIF)基因启动子区-173微卫星多态性与冠心病的相关性。方法 根据冠脉造影结果,将347例住院患者分为对照组229例和冠心病组118例,采用酶联免疫吸附试验(ELISA)检测血清MIF浓度,应用聚合酶链反应(PCR)技术及DNA测序技术分析基因。结果 (1)173C等位基因在冠心病组中频率较高。(2)173C等位基因的血清MIF浓度较173G等位基因高,173C/C基因型的血清MIF浓度较173C/G和173G/G基因型高。结论 本研究发现MIF-173G/C基因多态性可能与冠状动脉粥样硬化性心脏病的发展有关。Objective We analyzed the relationship of-173G/C MIF polymorphisms with soluble MIF in Coronary Atherosclerotic Disease（CAD） patients. Methods A total of 347 patients were selected,of which 229 normal-coronary and 118 Coronary artery disease subjects. Genotyping of-173G/C polymorphisms were performed by PCR and DNA sequencing. Serum MIF levels were measured using an ELISA kit. Patients were classified by coronary angiogram. Results （1）The frequency of the C genotype was higher in CAD patients than in the control.（2） Serum MIF levels was higher in 173C subjects than in 173G subjects. In addition,we found an increase in serum MIF levels in carriers of the（C/C） genotypes the-173 MIF polymorphisms. Conclusion These data suggest that MIF-173G/C polymorphism may be related to the development of CAD in a Chinese population.

关 键 词：巨噬细胞移动抑制因子 启动子 基因多态性 冠状动脉粥样硬化性心脏病 

分 类 号：R541.4[医药卫生—心血管疾病]