鹅细小病毒安徽分离株NS和VP基因的克隆及序列分析  

Cloning and Sequence Analysis of NS and VP Genes of Goose Parvovirus Strain in Anhui

作　　者：戴银[1] 张丹俊[1] 赵瑞宏[1] 胡晓苗[1] 沈学怀[1] 侯宏艳[1] 潘孝成[1] 周学利[1] DAI Yin;ZHANG Danjun;ZHAO Ruihong;HU Xiaomiao;SHEN Xuehuai;HOU Hongyan;PAN Xiaocheng;ZHOU Xueli(Institute of Animal Husbandry and Veterinary Science,Anhui Academy of Agricultural Science,Hefei 230031,China)

机构地区：[1]安徽省农业科学院畜牧兽医研究所,合肥230031

出　　处：《中国畜牧兽医》2018年第5期1177-1183,共7页China Animal Husbandry & Veterinary Medicine

基　　金：安徽省农科院人才发展专项(17F0404);安徽省农科院院所共建团队项目(18C0430);安徽省家禽产业技术体系资金项目

摘　　要：为研究安徽省鹅细小病毒(goose parvovirus,GPV)的遗传变异特征,本研究通过PCR扩增获得GPV基因AH,并与天鹅、番鸭、雁鹅等宿主的GPV相应基因序列进行了比对。序列分析可见,AH基因包括完整的NS和VP基因,长度分别为1 884和2 199bp,分别编码2个非结构蛋白和3个结构蛋白。遗传进化分析显示,安徽分离株AH基因与重庆分离株毒株RC16及安徽分离的强毒株Y、E、Yan-2等基因序列的遗传距离较近;而与安徽分离的鸭源细小病毒株DS15和匈牙利分离株Virulent B,尤其是与疫苗株SYG61v遗传距离较远。同源性分析结果表明,AH-NS基因与毒株RC16核苷酸序列同源性最高,为99.6%;与疫苗株SYG61v同源性最低,为94.3%;AH-VP基因与毒株RC16的核苷酸序列一致,而与毒株DS15同源性仅为95.2%。安徽GPV AH基因序列与强毒株的基因位点一致,在VP3基因的第797、1 141、1 144、1 169和1 185bp处分别为G、A、G、C、T,结合该毒株引发疾病的严重程度,符合高致病力毒株特点。本试验结果表明,GPV同源性普遍较高,宿主范围较广泛,对不同品种水禽均有较强的侵染力。To investigate the variation status of goose parvovirus（GPV）gene in Anhui,the gene of GPV was cloned by PCR,which we named as AH gene,and we compared AH gene with GPV genes of swan,Muscovy ducks,Yan goose and so on.The results showed that Anhui GPV AH gene included the complete NSand VP genes,which were 1 884 and 2 199 bp in length,and coding two nonstructural proteins and three structural proteins,respectively.Evolution analysis revealed that AH gene and Chongqing isolate RC16,Anhui isolates Y,E and Yan-2 had a close genetic relationship.The duck parvovirus strain DS15 isolated from Anhui and the virulent B strain of Hungary,in particular the vaccine strain SYG61 v,were far away from AH gene.Similarity analysis showed that the nucleotide homology between AH-NS gene and the isolate RC16 was highest（99.6%）.It showed the lowest homology between AH-NS gene and the vaccine strain SYG61 v,which was 94.3%.The nucleotide sequences of AH-VPand the isolate RC16 were completely consistent.The homology was 95.2% between AH-VPgene and the isolate DS15.The nu-cleotide sites of the VP3 gene 797,1 141,1 144,1 169 and 1 185 bp were G,A,G,C and T in GPV AH gene and virulent strains,respectively.And the Anhui GPV isolate was considered as a virulent strain with the characteristics of the severe infection.The results showed that the GPV had the higher homology and a wide host range,the different waterfowls could be infected.

关 键 词：鹅细小病毒(GPV) 非结构蛋白 结构蛋白 序列分析 

分 类 号：S852.65[农业科学—基础兽医学]