熔融聚合氨基酸的体外生物活性  

In vitro Bioactivity of Poly(amino acids) Synthesized by Melting Polycondensation

作　　者：樊晓霞 严永刚[2] FAN Xiao-xia1,2,YAN Yong-gang2(1. Medical College, Yan＇an University, Yan＇an 716000, Shaanxi, China;2. College of Physical Science and Technology,Sichuan University, Chengdu 610064, Chin)

机构地区：[1]延安大学医学院,陕西延安716000 [2]四川大学物理科学与技术学院,成都610064

出　　处：《功能高分子学报》2018年第3期279-284,共6页Journal of Functional Polymers

基　　金：延安大学博士科研启动项目(YDBK2016-09);四川省科技计划项目(2014GZX0010)

摘　　要：通过熔融缩聚的方法制备了以6-氨基己酸为主链的六元和四元氨基酸聚合物。采用傅里叶转换红外光谱(FT-IR)、X射线衍射(XRD)、差示扫描量热(DSC)、热重(TG)等分析方法对合成的两种氨基酸聚合物进行了理化性能表征。将氨基酸聚合物、聚酰胺和聚乙烯分别浸泡于模拟体液(SBF)中5d后,采用扫描电子显微镜(SEM)和X射线能谱(EDS)仪对样品的体外生物活性进行表征。结果表明:六元和四元氨基酸聚合物均为酰胺键结构,是半结晶型聚合物,具有较好的热稳定性、可加工性和良好的体外生物活性,它们的Ca和P元素的质量分数比值分别为2.58和2.94,而聚酰胺和聚乙烯无体外生物活性。The hexa-poly（amino acids）and tetra-poly（amino acids）based on 6-aminohexanoic acid were synthesized by in-situ melting polycondensation.In vitro bioactivity of hexa-poly（amino acids）and tetrapoly（amino acids）were characterized in comparison with polyamide and polyethylene.Fourier Transform Infrared Spectoscopy（FT-IR）、X-ray Diffraction（XRD）、Differential Scanning Calorimetry（DSC）and Thermal Gravity（TG）analysis of hexa-poly（amino acids）and tetra-poly（amino acids）were characterized for evaluating their chemical features.Scanning Electron Microscopy（SEM）and Energy-Dispersive X-ray Spectroscopy（EDS）of hexa-poly（amino acids）、tetra-poly（amino acids）、polyamide and polyethylene were tested after soaking into simulated body fluid（SBF）for 5 dfor assessing apatite forming in their surface.Results showed that hexa-poly（amino acids）and tetra-poly（amino acids）possessed amide band were both semi-crystallization structure.Peak crystallization temperatures of hexa-poly（amino acids）and tetrapoly（amino acids）were 159.5 ℃ and 157.6 ℃,respectively.Melting temperature of hexa-poly（amino acids）and tetra-poly（amino acids）were 205.9 ℃and 204.4 ℃,respectively.The total mass loss of tetrapoly（amino acids）and hexa-poly（amino acids）were 98.5% and 98.0% at about 800 ℃,respectively.SEM and EDS results showed that hexa-poly（amino acids）and tetra-poly（amino acids）induced the form of apatite on their surface after soaking into SBF for 5 d,showing good bioactivity.However,polyethylene and polyamide were not bioactive.w（Ca）/w（P）for hexa-poly（amino acids）and tetra-poly（amino acids）were 2.58 and 2.94,respectively.

关 键 词：六元聚氨基酸 四元聚氨基酸 聚乙烯 聚酰胺 体外生物活性 

分 类 号：R318.08[医药卫生—生物医学工程]