196份福建葡萄品种资源亲缘关系的ISSR分析  

Genetic Relationship Among Grape Varieties in Fujian by ISSR Markers

作　　者：张富民 赖呈纯[2] 黄贤贵[2] 范丽华[2] 潘红[2] 谢鸿根[2] ZHANG Fu-rain;LAI Cheng-chun;HUANG Xian-gui;FAN Li-hua;PAN Hong;XIE Hong-gen(Fuan agricultural bureau, Fuzhou, Fuan 355000, China;Institute of Agricultural Engineering and Technology, FujianAcademy of Agricultural Sciences, Fuzhou, Fuan 350003, China)

机构地区：[1]福建省福安市农业局,福建福安355000 [2]福建省农业科学院农业工程技术研究所,福建福州350003

出　　处：《福建农业学报》2018年第3期263-268,共6页Fujian Journal of Agricultural Sciences

基　　金：福建省科技计划项目--省属公益类科研院所基本科研专项(2016R1014-1);福建省自然科学基金项目(2016J01126)

摘　　要：利用ISSR-PCR分子标记技术对福建省各葡萄种植区收集到的196份葡萄资源进行亲缘关系鉴定。试验结果表明,12条引物可以清晰扩增出164条谱带,多态性位点的平均百分率为91.44%;聚类分析表明,196份葡萄资源可以分为3大类,即东亚种群、欧美杂种和欧亚种;群体遗传分析表明,葡萄资源种内存在丰富的遗传多样性。Genetic relationships among 196 grape cultivars collected from various regions in Fujian were determined using ISSR-PCR.The 12 selected primers amplified 164 bands from the plants with up to 91.44% polymorphic loci.The clustering analysis revealed that the germplasms could be divided into three categories as the eastern Asia population,the inter-specific hybrids（Vitis vinifera V.labrusca）and V.vinifera.A population genetic polymorphism analysis on the cultivars showed abundant diversities among them.

关 键 词：葡萄 种质资源 ISSR 亲缘关系 聚类分析 

分 类 号：S663.1[农业科学—果树学]