双孢蘑菇As2796子实体发育转录组测序分析  

Transcriptome Sequencing on Fruiting Body of Agaricus bisporus in Developing Stages

作　　者：施肖堃[1] 蔡志欣[1] 郭仲杰[1] 卢园萍 陈美元[1] 廖剑华[1] 王泽生[1] SHI Xiao-kun;CAI Zhi-xin;GUO Zhong-jie;LU Yuan-ping;CHEN Mei-yuan;LIAO Jian-hua;WANG Ze-sheng(The Edible Fungi Institute of Fujian Academy of Agricultural Sciences, National and Local Joint Engineering Research Center for Breeding ＆ Cultivation of Featured Edible Fungi, Fuzhou, Fujian 350014, China)

机构地区：[1]福建省农业科学院食用菌研究所/特色食用菌繁育与栽培国家地方联合工程研究中心,福建福州350014

出　　处：《福建农业学报》2018年第3期282-287,共6页Fujian Journal of Agricultural Sciences

基　　金：福建省科技计划项目--省属公益类科研院所基本科研专项(2017R1020-4;2015R1020-1);国家现代农业产业技术体系专项(CARS20);福建省种业创新与产业化工程项目(fjzycxny2017009);福建省农业科学院科技创新团队建设项目(STIT2017-1-6)

摘　　要：对双孢蘑菇As2796子实体发育4个不同阶段样品的转录组进行测序,并与双孢蘑菇参考基因组进行序列比对。共比对到9 207个转录本,发掘新基因393个,其中164个得到功能注释。基于比对结果进行各基因在不同样品中的表达量分析,识别差异表达基因,发现原基期与其他3个不同发育时期有316个共同差异基因。对差异基因进行功能注释和富集分析,可为进一步研究双孢蘑菇子实体生长发育的相关基因奠定基础。Samples of Agaricus bisporus As2796 fruiting bodies in 4 developmental stages were collected for transcriptome sequencing.After aligned with the reference genome sequence of the mushroom,9 207 transcripts were identified.Among them,393 were new genes with 164 being functionally annotated.The expression level of the genes was analyzed to differentiate the samples.The alignments showed an overlapping of 316 differentially expressed genes between the primordium and the 3 other developmental stages.An analysis and function evaluation on these genes were performed for further study on the growth and development of A.bisporus fruiting body.

关 键 词：双孢蘑菇 子实体发育 转录组测序 

分 类 号：S646.11[农业科学—蔬菜学]