不同月龄阶段陕北白绒山羊生精细胞发育规律研究  

Histological Analysis of Testis in Shanbei White Cashmere Goats at Different Developmental Stages

作　　者：韩聪[1] 方乾[1] 李浩[1] 胡姗 朱海鲸 胡建宏[1] HAN Cong;FANG Qian;LI Hao;HU Shan;ZHU Haijing;HU Jianhong(College of Animal Science and Technology,Northwest A＆F University,Yangling,Shaanxi 712100;College of Life Sciences,Yulin University,Yulin, Shaanxi 719000)

机构地区：[1]西北农林科技大学动物科技学院,陕西杨凌712100 [2]榆林学院生命科学学院,陕西榆林719000

出　　处：《家畜生态学报》2018年第4期43-48,共6页Journal of Domestic Animal Ecology

基　　金：国家自然科学基金项目(31672425)

摘　　要：为研究陕北白绒山羊生精细胞发育规律,取1月龄、3月龄、6月龄及成年陕北白绒山羊睾丸组织,HE染色观察睾丸发育情况。结果表明:(1)随着年龄的增长,曲细精管直径显著增大(P<0.05),6月龄达到150.72μm,略低于成年羊(161.94μm)(P>0.05)。(2)细胞层数随年龄的增长而增长,6月龄达到7.70层,显著高于1月龄和3月龄(P<0.05)。曲细精管横截面细胞总数随年龄增长而增长,6月龄和成年绒山羊曲细精管横截面细胞总数显著高于1月龄和3月龄绒山羊(P<0.05)。(3)3月龄陕北白绒山羊曲细精管中精原细胞最为丰富,占总细胞数的46.40%,明显高于1月龄(33.10%)、6月龄(9.10%)和成年羊(7.60%)(P<0.05)。(4)精原干细胞标记基因(CD49f、PLZF、Thy1、ETV5、VASA、Oct4、Nanog、Nanos2)均在3月龄睾丸中高表达,其中Oct4、PLZF、Thy1、ETV5、VASA、Nanog及Nanos2显著高于1月龄、6月龄及成年羊(P<0.05)。研究结果表明,6月龄陕北白绒山羊已经达到性成熟;与1月龄、6月龄和成年羊相比,3月龄陕北白绒山羊睾丸组织含有更加丰富的精原细胞。In order to explore the spermatogenic cell development of Shaanbei white cashmere goats at different developmental stages,the testis of Shaanbei white cashmere goats（one-,three-,six-month-old and adult goats）were collected and cut into pieces for staining using hematoxylin-eosin.The results showed that（1）The diameter of goat seminiferous tubules increased significantly with age（P〈0.05）.The difference in diameter between 3-month-old and adult goats was not significant,representing 150.72μm and 161.94μm,respectively（P〉0.05）.（2）The cell layer of goats also increased with age,the cell layer of 6-month-old goat（7.70 layers）was significantly higher than that of one-and three-month-old goats,respectively（P〈0.05）.The total number of cross-sectional cells in seminiferous tubules both of six-month-old and adult goats was strikingly different compared to those of 1-month-old and 3-month-old cashmere goats（P〈0.05）.（3）Moreover,the proportion of spermatogonia of 3-month-old goats（46.40%）was significantly higher than that of one-,six-month-old and adult goats,just about 33.10%,9.10% and 7.60%,respectively（P〈0.05）.（4）The expression of PLZF in testis decreased significantly with age（P〈0.05）.Marker genes of SSCs,such as CD49 f,PLZF,Thy1,ETV5,VASA,Oct4,Nanog and Nanos,were highly expressed in the testis of 3-month-old goats,and except for the CD49 fgene,expression levels of other genes were significantly higher than those of other groups（P〈0.05）.The results showed that the cashmere goats reached sexual maturity in 6 month old,and the most abundant spermatogonia were in 3-month-old testis.

关 键 词：陕北白绒山羊 生精细胞 发育规律 精原细胞 

分 类 号：S811.5[农业科学—畜牧学]