百蕊草亲水性化学成分研究  

Study on hydrophilic chemical constituents from Thesium Chinensis Turcz

作　　者：宣伟东 范正平 胡水根 徐德东 XUAN Weidong;FAN Zhengping;HU Shuigen;XU Dedong(No .411 Hospital of PLA ,Shanghai 200081 ,China)

机构地区：[1]解放军411医院药学科,上海200081

出　　处：《药学实践杂志》2018年第3期270-273,共4页Journal of Pharmaceutical Practice

基　　金：上海市卫生局局级科研课题(2012085)

摘　　要：目的对百蕊草中亲水性化学成分进行研究。方法采用硅胶柱色谱、SephadexLH-20凝胶柱色谱等分离手段,对百蕊草水提取物经AB-8大孔树脂吸附,50%乙醇洗脱的亲水性组分(TT50)进行分离纯化,应用核磁共振波谱分析、结合文献报道鉴定化合物结构。结果从TT50中分离纯化得到6个化合物,分别鉴定为:山奈酚(1),紫云英苷(2),山奈酚-3,7-二-O-β-D-吡喃葡萄糖苷(3),山奈酚-3-O-L-吡喃鼠李糖基(1→2)-β-D-吡喃葡萄糖苷(4),山奈酚-3-O-L-吡喃鼠李糖基(1→2)-[6-O-乙酰基]-β-D-吡喃葡萄糖苷(5),芸香苷(6)。结论 TT50中主要成分为以山奈酚为母核的黄酮苷类化合物,化合物4、5为首次从百蕊草中分离得到。Objective To investigate the hydrophilic chemical constituents from Thesium Chinensis Turcz.MethodsThe water extract of Thesium Chinensis Turcz.was adsorbed onto AB-8 resin column and eluted with 50% ethanol to give the hydrophilic portion TT50.TT50 was further separated and purified by repeated column chromatography on silica gel,Sephadex LH-20.The structures of these purified compounds were identified by NMR spectral analysis and comparison with the reported data.Results Six compounds were isolated and identified as kaempferol（1）,kaempferol-3-O-glucoside（2）,kaepmferol-3,7-diO-β-D-glucopyranoside（3）,kaempferol-3-O-L-rhamnopyranosyl（1→2）-β-D-glucopyranoside（4）,kaemperol-3-O-α-L-rhamnopyranosyl（1→2）-[6-O-acetyl]-β-D-glucopyranoside（5）,rutinoside（6）.Conclusion The main constituents of TT50 were kaempferol glycosides.Compounds 4 and 5 were isolated from this plant for the first time.

关 键 词：百蕊草 化学成分 黄酮苷 

分 类 号：R284.1[医药卫生—中药学]