发烟罐燃烧烟雾吸入致大鼠急性肺损伤模型的研究  

Study on the acute lung injury rat model induced by inhalation of smoke from burning smoke pot

作　　者：李佳 詹球[2] 邓春江 崔培 童亚林[1,2] Li Jia1,2, Zhan Qiu2, Deng Chunjiang2, Cui Pei2, Tong Yalin1,2(1. College of Life Science, Guangxi Normal University, Guilin, Guangxi 541002, China; 2. Research Laboratory of Burns and Trauma, Department of Burns and Trauma Orthopedics, 181^st Hospital of Chinese PLA, Guilin, Guangxi 541002, Chin)

机构地区：[1]广西师范大学生命科学学院,桂林541002 [2]解放军第一八一医院烧伤整形与创面修复外科烧创伤研究实验室,广西桂林541002

出　　处：《感染．炎症．修复》2017年第4期199-204,F0003,共7页Infection Inflammation Repair

基　　金：院士工作站专项基金;漓江学者专项基金;桂林市科技计划项目(20170109-35);广西科技计划项目(1140003A-39)

摘　　要：目的:探讨发烟罐烟雾(白烟)吸入致大鼠急性肺损伤模型的建立方法。方法:228只SD大鼠随机分为白烟吸入0 min组(对照组,n=12)、3 min组(W3组,n=72)、5 min组(W5组,n=72)、7 min组(W7组,n=72)。大鼠置于自制的多功能烟雾致伤实验设备中持续不同时间吸入发烟罐烟雾,观察伤后72 h大鼠存活率以及伤后6、12、24、48、72 h肺组织病理学变化、湿/干重比值、氧合指数(动脉血氧分压/吸入氧浓度,PaO_2/FiO_2)和二氧化碳分压(PaCO_2)的变化。结果:大鼠肺组织HE染色结果显示,伤后48 h内,随着烟雾暴露时间和观察时间的延长,大鼠气道黏膜与肺泡间隔的充血、水肿、出血和炎性细胞浸润呈进行性加重,肺组织损伤病理评分逐渐增高(P<0.05)。72 h后,W3组水肿和炎性分泌物较之前减少,病理评分显著降低(P<0.05),W5和W7组肺泡水肿和炎性细胞浸润更加明显,病理评分结果仍显著增加(P<0.05)。伤后48 h内,3组大鼠肺组织湿/干重比值随着观察时间延长而逐渐升高(P<0.05)。伤后6 h,3个致伤组大鼠氧合指数均低于对照组(P<0.05),伤后24 h W7组和伤后48 h的W5组大鼠氧合指数<300 mmHg;伤后72 h,W5组氧合指数<200 mm Hg。除W3组大鼠致伤后PaCO_2稍增高外,其他致伤组大鼠在伤后6 h PaCO_2即显著增高(P<0.05),72 h升至最高值。W3组大鼠72 h存活率为83.3%,W5组为75.0%,W7组仅为25.0%。结论:吸入烟雾3 min和5 min分别可作为大鼠轻度和重度ALI模型,而吸入烟雾7 min大鼠因其死亡率较高,作为致伤模型时需谨慎。Objective： To study the modeling method of acute lung injury（ALI） rat model induced by inhalation of smoke from burning smoke pot. Methods： Two hundred and twenty-eight rats were randomly divided into 4 groups： inhalation of white smoke for 0 minute group（C group, n=12）, 3 minutes group（W3 group, n=72）, 5 minutes group（W5 group, n=72） and 7 minutes group（W7 group, n=72）. Make rats continuously inhale the white smoke for different time in a self-made multifunction smoke vulnerate experimental instrument. The survival rate of rats in each group was respectively counted 72 hours after treatment, and the histopathological changes of lung tissue, ratio of wet/dry weight, oxygenation index（PaO2/FiO2） and partial pressure of carbon dioxide（PaCO2） were observed. Results： HE staining of lung tissue showed that, 48 hours post-injury, the hyperemia, edema, hemorrhage and inflammatory cell infiltration in rats＇ airway mucosa and alveolar septum were increased progressively with the increased time of smoke exposure and observation, and the pathological score of lung tissue was also increased（P〈0.05）. After 72 hours, the edema and inflammatory secretion decreased and the pathological score was also significantly reduced in W3 group（P〈0.05）, while the alveolar edema and inflammatory infiltration were more obvious, and the pathological score increased significantly in W5 group and W7 group（P〈0.05）. Within 48 hours post-injury, the ratio of wet/dry weight of lung tissue in the 3 vulnerate groups increased gradually with the increased time of observation（P〈0.05）. The oxygenation index of rats in the 3 vulnerate groups were all lower than that in group C at 6 hours after injury（P〈0.05）. The oxygenation index was less than 300 mmHg in W5 group at 24 hours post-injury and W7 group at 48 hours post-injury. At 72 hours post-injury, the oxygenation index was less than 200 mmHg in W5 group. Except the PaCO2 slightly increased in W3 group, the PaCO2 increased obviously in

关 键 词：白烟 发烟罐 烟雾吸入 肺损伤 急性 

分 类 号：R563[医药卫生—呼吸系统]