食源性腹泻副溶血弧菌分离株调查分析  

作　　者：强鑫华 周丽华[1] 王萍[1] 柳秋霞 

机构地区：[1]浙江省湖州市第一人民医院,313000

出　　处：《浙江临床医学》2018年第5期971-972,共2页Zhejiang Clinical Medical Journal

基　　金：“十二五”国家科技重大专项（2012ZX10004-210）;浙江省医药卫生科技项目（2016KYA171）;浙江省湖州市科技局项目（2017GY40）

摘　　要：目的 分析食源性腹泻患者副溶血弧菌（VP）季节分布及感染患者年龄特征、毒力基因、血清学分型.方法 对2014年1月至2016年12月急性腹泻患者粪便分离的125株副溶血弧菌进行常规培养、血清分型及PCR毒力基因检测.结果 副溶血弧菌感染发生有明显的季节性,夏秋季高发,尤以6～10月份为高峰期.感染患者中21～30岁占37.6％,31～40岁占24.8％.分离得到17个血清型,其中O3：K6占59.2％.经单重PCR进行毒力基因检测显示：tdh＋/trh-分离株所占比例为88.0％,tdh-/trh-分离株为6.40％,检测到2株携带（tdh＋/trh＋）基因.结论 湖州地区副溶血性弧菌感染发生呈明显的季节分布特征,且感染的患者以青壮年居多,优势血清型为O3：K6;多数菌株携带tdh＋/trh-毒力基因.Objective To analyze the seasonal distribution of Vibrio parahaemolyticus （ VP ） and the age characteristics, virulence genes and serotypes of patients with food-borne diarrhea. Methods 125 strains of Vibrio parahaemolyticus isolated from feces of acute diarrhea patients from January 2014 to December 2016 were routinely cultured, serotypes and PCR virulence genes were tested. Results Vibrio parahaemolyticus infection occurred obvious seasonal, the most high incidence in summer and autumn, especially in 6 to 10 months as the peak. Infected patients ranged from 21 to 30 years old, accounted for 37.6%, 31 to 40 years followed, accounted for 24.8%. 17 serotypes were isolated, of which 03：K6 was the most, accounted for 59.2%. Virulence genes detected by single-PCR showed that tdh ＋ / trh- accounted for the highest proportion （ 88.0% ） and tdh- / trh- （ 6.40% ） . Two tdh ＋ / trh- genes were found. Conclusion The distribution ofVibrio parahaemolyticus in Huzhou is obviously seasonal distribution. Most infected patients are young and middle-aged, and the predominant serotype is 03 ： K6. Most strains carries tdh ＋ / trh- virulence genes.

关 键 词：副溶血弧菌 季节性分布 血清分型 毒力基因 

分 类 号：R378.3[医药卫生—病原生物学]