电针“中髎”“天枢”改善早发性卵巢功能不全模型大鼠卵巢功能的实验研究  

Effects of electroacupuncture at “Zhongliao”(BL 33) and “Tianshu”(ST 25) on ovarian function in rats with premature ovarian insufficiency

作　　者：王伟明[1] 王扬[1] 吴佳霓[1] 杨丽坤 刘志顺[1] WANG weiming, WANG Yang, WU Jiani, YANG Likun, LiU Zhishun(Department of Acupuncture and Moxibustion, Guang＇anmen Hospital, China Academy of Chinese Medical Sciences, Beijing 100053, Chin)

机构地区：[1]中国中医科学院广安门医院针灸科,北京100053

出　　处：《中国针灸》2018年第5期519-526,共8页Chinese Acupuncture & Moxibustion

基　　金：中国中医科学院广安门医院所级课题:2016 S 357

摘　　要：目的:探讨电针"中髎""天枢"穴对早发性卵巢功能不全(POI)模型大鼠卵巢功能的影响。方法:将动情周期规则的48只SD雌性大鼠分为空白组、模型组、电针组、捆绑组、他莫昔芬组,除空白组8只外,其余各10只。模型组、电针组、捆绑组、他莫昔芬组使用去氧乙烯基环己烯(VCD)造模,160 mg/kg,腹腔注射,连续15 d。空白组在造模期间给予正常饲养。造模成功当天,电针组使用"中髎""天枢"穴进行电针干预(连续波,1~3 Hz,0.1~1 mA),每次20 min,前2周每日1次,每周5次,后2周隔日1次,每周3次;他莫昔芬组使用枸橼酸他莫昔芬(1 mg/kg,每次注射总体积0.3~0.5 mL)皮下注射,前2周每日1次、每周5次,后两周隔日1次,每周3次;捆绑组同电针组捆绑固定;空白组、模型组正常饲养。干预4周后检测各组大鼠性腺湿重和性腺指数,采用酶联免疫吸附(ELISA)法检测血清抗苗勒激素(AMH)、抑制素B(inhibin B),观察卵巢组织形态、计数原始卵泡、初级卵泡、窦状卵泡和闭锁卵泡数量,检测卵巢组织胰岛素样生长因子1(IGF-1)和胰岛素样生长因子1受体(IGF-1R)m RNA相对表达量。结果:(1)干预后,与空白组比较,模型组、电针组、捆绑组和他莫昔芬组大鼠左、右侧卵巢湿重、卵巢指数、子宫湿重和子宫指数均明显降低(均P<0.01),但电针组、捆绑组和他莫昔芬组与模型组比较差异均无统计学意义(均P>0.05)。(2)干预后,与空白组比较,模型组、电针组、捆绑组和他莫昔芬组大鼠血清AMH和inhibin B水平明显降低,血清FSH、LH水平升高,E_2水平降低(均P<0.01);与模型组比较,电针组、他莫昔芬组大鼠血清AMH和inhibin B水平明显升高、血清FSH、LH水平明显降低,E_2水平明显升高(均P<0.01)。(3)干预后,与空白组比较,模型组、电针组、捆绑组和他莫昔芬组大鼠卵巢颜色暗红苍白,周围散在或不存在颗粒状小体,形态较小,呈萎缩状;原始卵泡明显减少,甚�Objective To explore the effects of electroacupuncture（EA） at ＂Zhongliao＂（BL 33） and ＂Tianshu＂（ST 25） on ovarian function in rats with premature ovarian insufficiency（POI）. Methods A total of 48 SD female rats with regular estrus were divided into a blank group（n=8）, a model group（n=10）, an EA group（n=10）, a binding group（n=10） and a tamoxifen（TAM） group（n=10）. The rats in the model group, EA group, binding group and TAM group were all treated with intraperitoneal injection of 4-vinylcyclohexene diepoxide（VCD, 160 mg/kg） for 15 consecutive days to establish the model of POI; the rats in the blank group were treated with normal diet. After the model was established successfully, the rats in the EA group were treated with EA at ＂Zhongliao＂（BL 33） and ＂Tianshu＂（ST 25） with continuous wave（1 to 3 Hz, 0.1 to 1 mA） for 20 minutes,once a day（five times a week） for the first two weeks and once every other day（three times a week） for the following two weeks.The rats in the TAM group were treated with subcutaneous injection of tamoxifen（1 mg/kg）, once a day（five times a week） for the first two weeks and once every other day（three times a week） for the following two weeks. The rats in the binding group were bound by a small sack as the EA group. The rats in the blank group and the model group were treated with normal diet.After four weeks, the sexual gland weight and index were tested in each group; the ELISA method was applied to test the level of anti-mllerian hormone（AMH） and inhibin B; the morphology of ovary was observed; the number of primordial follicles, primary follicle, antral follicle and atretic follicle was counted; the expression of insulin-like growth factor-1（IGF-1） and insulin-like growth factor-1 receptor（IGF-1 R） were measured. Results（1） Compared with the blank group, the ovary weight, ovary index,uterus weight and uterus index were significantly decreased after treatment in the model group, EA gro

关 键 词：早发性卵巢功能不全 电针 穴 中髎 穴 天枢 胰岛素样生长因子1 胰岛素样生长因子1受体 

分 类 号：R245.97[医药卫生—针灸推拿学]