人脂肪基质血管成分上清液修复急性肾缺血再灌注损伤  

Protection of human adipose stromal vascular fraction derived culture supernatant against acute renal ischemia/reperfusion injury

作　　者：周六化[1] 申江伟 许露伟[1] 宋群[1] 吴然[1] 葛余正[1] 徐郑[1] 朱佳庚[1] 窦全亮[1] 贾瑞鹏[1] Zhou Liuhua;Shen Jiangwei;Xu Luwei;Song Oun;Wu Ran;Ge Yuzheng;Xu Zheng;Zhu Jiageng;Dou Quanliang;Jia Ruipeng(Department of Urology, Nanjing First Hospital, Nanjing Medical University, Nanjing 210006, China)

机构地区：[1]南京医科大学附属南京医院(南京市第一医院)泌尿外科,210006

出　　处：《中华实验外科杂志》2018年第5期874-876,共3页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金（81570613、31500785）;江苏省卫计委科教强卫工程青年医学人才项目（QNRC2016071）

摘　　要：目的 观察人脂肪基质血管成分（SVF）上清液对急性肾缺血再灌注损伤（IRI）的影响.方法 分离制备SVF上清液（SVF-CM）,建立肾小管上皮细胞（HK-2）低氧、复氧损伤模型,进行细胞增殖、凋亡检测.将32只SD大鼠随机分成4组,即SVF-CM组、SVF组、对照组及假手术组.术后24 h收集血液及肾组织标本,行血清肌酐（SCr）、尿素氮（BUN）及苏木素-伊红（HE）、免疫组织化学检测.结果 SVF-CM与SVF均显著促进HK-2增殖、抑制HK-2凋亡,两组之间增殖（P=0.781）、凋亡（P =0.921、0.280）比较差异无统计学意义.术后24 h,SVF-CM、SVF组SCr和BUN水平显著低于对照组（P =0.000）,但两组间比较差异无统计学意义（P=0.993、0.985）.SVF-CM、SVF组肾小管损伤评分为（1.9±0.8）、（1.7±0.9）分,低于对照组[（3.3±0.7）分,P=0.000];增殖细胞核抗原（PCNA）阳性细胞数为（60.86±18.39）、（62.51±20.03）个,高于对照组[（33.61±6.91）个,P=0.000];凋亡细胞数为（24.73 ±6.82） 、（22.11 ±5.68）个,低于对照组[（45.12±7.85）个,P =0.000].但比较SVF-CM组和SVF组肾小管损伤评分、PCNA阳性细胞数以及凋亡细胞数,差异无统计学意义（P=0.114、0.875、0.197）.结论 SVF上清液可促进大鼠肾IRI结构和功能恢复,且与SVF具有相同作用效果.Objective To observe the effect of human adipose stromal vascular fraction （SVF） derived culture supernatant protecting kidney from acute ischemia/reperfusion injury （IRI）.Methods SVF was isolated and was used for the preparation of SVF derived culture supernatant named SVF conditioned medium （SVF-CM）.Hypoxia/reoxygenation （H/R） injury model of renal tubular epithelial cell （HK-2） was established for cell proliferation and apoptosis assay.Thirty-two SD rats were divided into four groups randomly：SVF-CM group,SVF group,control group,and sham group.Twenty-four hours after surgery,blood sample was collected for the detection of serum creatinine （SCr） and blood urea nitrogen （BUN）.Kidney tissues were retrieved for hematoxylin and eosin （HE） and immunohistochemical staining.Results Both SVF-CM and SVF could significantly enhance the proliferative capability as well as attenuate the apoptosis of HK-2,while no significant difference could be found between the two groups for cell proliferation （P =0.781） and apoptosis （P =0.921,0.280）.At 24 h after surgery,serum levels of SCr and BUN in both SVF-CM and SVF groups were significantly lower than that in control group （P =0.000）.However,there was no significant difference between the two groups （P =0.993,0.985）.Tubular damage score in both SVF-CM （1.9 ± 0.8） and SVF （1.7 ± 0.9） groups were significantly lower than that in control group （3.3 ± 0.7） （P =0.000）.Meanwhile,the numbers of proliferating cell nuclear antigen （PCNA） positive cells in both SVF-CM [（60.86 ± 18.39） cells] and SVF [（62.51 ±20.03） cells] groups were significantly higher than that in control group [（33.61 ± 6.91） cells] （P =0.000）.Additionally,the numbers of terminal-deoxynucleotidyl transferase mediated nick end labeling （TUNEL） positive cells in both SVF-CM [（24.73 ± 6.82） cells] and SVF [（22.11 ± 5.68） cells] groups were significantly lower that in control group [（45.12 ± 7.85） cells] （P =0.0

关 键 词：脂肪基质血管成分 细胞上清液 急性肾损伤 缺血再灌注损伤 

分 类 号：R692[医药卫生—泌尿科学]