过敏洗剂的高效液相色谱指纹图谱研究  

Study on HPLC Fingerprint Chromatogram of Guomin Lotion

作　　者：肖淼生[1] 朱海涛[1] 张幼林[1] 尹聪聪 黄良永[1] Xiao Miaosheng;Zhu Haitao;Zhang Youlin;Yin Congcong;Huang Liangyong(Shiyan Taihe Hospital Affiliated to Hubei University of Medicine, Hubei Shiyan 442000, China;College of Pharmacy, Hubei University of Medicine, Hubei Shiyan 442000, China)

机构地区：[1]湖北医药学院附属十堰市太和医院,湖北十堰442000 [2]湖北医药学院药学院,湖北十堰442000

出　　处：《儿科药学杂志》2018年第5期46-48,共3页Journal of Pediatric Pharmacy

摘　　要：目的:按照过敏洗浴方的生产工艺制备过敏洗剂,测定过敏洗剂的高效液相色谱(HPLC)指纹图谱,为质量控制提供参考。方法:色谱柱Waters Sunfire C18(250 mm×4.6 mm,5μm),乙腈为流动相A,0.1%磷酸溶液为流动相B,流速1.0 m L/min,检测波长254 nm,柱温30℃,进样量10μL,采用HPLC二元梯度洗脱法绘制过敏洗剂的指纹图谱。结果:11批过敏洗剂标定15个共有峰,指认5个已知峰为绿原酸、升麻苷、木犀草苷、5-O-甲基维斯阿米醇苷和甘草酸。结论:本研究建立的过敏洗剂HPLC指纹图谱测定方法具有较好的专属性、重复性和实用性,色谱峰信息量大,为全面控制制剂的质量提供了可靠的方法。Objective： To measure the HPLC fingerprint chromatogram of Guomin lotion,provide reference for quality control.Methods： The separation was performed on a Waters Sunfire C18 column（ 250 mm × 4. 6 mm,5 μm）,eluted with the mobile phase consisting of acetonitrile-0. 1% phosphoric acid,the flow rate was 1. 0 m L/min,the determine wavelength was 254 nm,the column temperature was 30 ℃. Results： The sample revealed that there were fifteen common peaks and five peaks were identificated to chlorogenic acid,prim-o-Gluccosylcimifugin,galuteolin,5-O-methylvisammioside,glycyrrhizic acid. Conclusion： This method of HPLC fingerprint chromatogram have good specificity,repeatability and practicability. There is much information in chromatogram peaks,which provides credible method for controlling the quality Guomin lotion.

关 键 词：过敏洗剂 高效液相色谱 指纹图谱 

分 类 号：R917[医药卫生—药物分析学]