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作 者:林文婷 高睿[1] 滕亮 王一霖 姚国泰 陈江汉[1] LIN Wenting, GAO Rui, TENG Liang, WANG Yilin, YAO Guotai, CHEN Jianghan.(Department of Dermatology, Changzheng Hospital, Second Military Medical University, Shanghai 200003, Chin)
机构地区:[1]第二军医大学长征医院皮肤科,上海200003
出 处:《中国感染与化疗杂志》2018年第3期306-308,共3页Chinese Journal of Infection and Chemotherapy
基 金:国家自然科学基金(81772158)
摘 要:目的建立成熟的大鼠新型隐球菌脑膜炎模型。方法将SD大鼠分成4组,脑池内分别接种1×10~5 cfu、1×10~6 cfu、1×10~7 cfu 3个梯度新型隐球菌负荷量及0.9% NaCl溶液,观察接种后大鼠的临床症状,在第14、21天取脑脊液做培养并计数,观察28 d内大鼠死亡情况。结果 1×10~5 cfu组无明显临床症状,菌负荷下降,无死亡;1×10~6 cfu组症状轻微,菌负荷略有上升,死亡率低;1×10~7 cfu有明显的临床症状,死亡率较高,菌负荷明显增加;0.9% NaCl溶液大鼠活动生长正常,无死亡,脑脊液培养阴性。结论 1×10~7 cfu脑池注射可出现明显的临床症状,28 d死亡率>80%,可构建较为理想的大鼠新型隐球菌脑膜炎模型。Objective To establish a rat model of Cryptococcus neoformans meningitis. Methods Sprague-Dawley rats were divided into four groups (group A to D). The load of Cryptococcus neoformans was inoculated by intracisternal injection to the animals in Group A (1×10^5 cfu), Group B (1×10^6 cfu), Group C (1×10^7 cfu), and 0.9% NaCl solution to Group D. The rats were observed after inoculation for their clinical symptoms. On day 14 and day 21 after inoculation, cerebrospinal fluid was sampled and cultured for counting of bacterial colonies. The 28-day mortality of the animals was calculated. Results All the animals in group A (1×10^5 cfu) survived without any apparent clinical symptoms, and associated with decreasing bacteria load. The animals in group B (1×10^6 cfu) had mild symptoms associated with low mortality rate and slightly increased bacterial load. The animals in group C (1×10^7 cfu) showed a lot of symptoms and associated with high mortality rate and significantly increased bacteria load. All the animals in group D (0.9% NaCl solution) survived with normal activity. No bacterial colony was cultured from the cerebrospinal fluid. Conclusions Intracisternal injection of 1×10^7 cfu Cryptococcus neoformans to rats could cause apparent clinical symptoms of meningitis. The 28-day mortality rate of such a rat model of Cryptococcus neoformans meningitis is greater than 80%. An ideal rat model of Cryptococcus neoformans meningitis is established successfully.
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