乌拉坦致C57BL/6J小鼠肺腺瘤动物模型方法研究  

Animal Model of Lung Adenoma in C57BL/6J Mice Induced by Urethane

作　　者：陈晨[1,2] 盛云华 王宇[2] 王巧旭[2] 谷舒怡[2] 胡玥[2] 唐黎明[2] CHEN Chen1,2, SHENG Yun-hua2, WANG Yu2, WANG Qiao-xu2, GU Shu-yi2, HU Yue2, TANG Li-ming2.(1. School of Pharmacy, Fudan University, Shanghai 201203, China; 2. Shanghai Institute for Food and Drug Control, Shanghai 201203, Chin)

机构地区：[1]复旦大学药学院,上海201203 [2]上海市食品药品检验所药理毒理室,上海201203

出　　处：《中国药学杂志》2018年第9期701-706,共6页Chinese Pharmaceutical Journal

摘　　要：目的采用3种不同的造模方法,比较并优化乌拉坦致C57BL/6J小鼠肺腺瘤动物模型建立的方法。方法取雌性C57BL/6J小鼠,分成4组,阴性对照组和模型Ⅰ组,模型Ⅱ组,模型Ⅲ组。模型Ⅰ组腹腔注射800 mg·kg-1乌拉坦,2次·周-1,连续5周;模型Ⅱ组腹腔注射1 000 mg·kg-1乌拉坦,2次·周-1,连续5周;模型Ⅲ组腹腔注射1 000 mg·kg-1乌拉坦,5 d·次-1,连续8次。阴性对照组给予等体积氯化钠注射液,给药方式与模型Ⅱ组相同。每周称量动物体重。所有组分别于第28周取主要脏器心、肝、脾、肺、肾、脑、胸腺,计算脏器指数,同时另取肺组织观察,计腺瘤数,量取肺腺瘤直径,并对肺、胸腺进行组织病理学检查。收集阴性对照组和模型组小鼠支气管肺泡灌洗液,对细胞进行分类计数分析。结果与阴性对照组相比,3种造模方法均100%诱导出肺腺瘤,模型Ⅰ组和模型Ⅲ组肿瘤数目均为6左右,模型Ⅱ组肿瘤数目大约为2。3组造模小鼠肺指数均增大,脑、脾、胸腺指数均减小,支气管肺泡灌洗液分析表明模型组淋巴细胞和多型核白细胞多于阴性对照组。结论 3种造模方法均100%诱导出肺腺瘤,综合检测各指标,模型Ⅰ组和Ⅲ组相对于模型Ⅱ组诱导肺肿瘤的数目相对较多,模型较稳定均一。OBJECTIVE To compare and optimize the animal model of lung cancer on C57BL/6J mice by three different experimental methods.METHODS Female C57BL/6J mice were divided into four groups：control group, model group Ⅰ, Ⅱ and Ⅲ. The mice in model groupⅠreceived 800 mg·kg^-1 urethane by intraperitoneal injection twice a week, followed by 5 weeks. The mice in model group Ⅱ were injected intraperitoneally with 1 000 mg·kg^-1 urethane in the same way with model group Ⅰ. The mice in model group Ⅲ received urethane at the dose of 1 000 mg·kg^-1 intraperitoneally, every 5 day continuing for 8 weeks. The control group was given equal volume of sodium chloride solutions in the same way as the model group Ⅱ. The main organs of all the groups were taken such as heart, liver, spleen, lung, kidney, brain and thymus at the 28th week, and their organs index was calculated. At the same time the lung tissues were examined to get the amount of adenomas and the diameters, and developed the lung and thymus histopathological examination. The bronchial alveolar lavage fluids were collected to classify the cells of the control and experimental groups.RESULTS Compared with the control group, all the three experimental groups could induce lung adenomas absolutely, the number of tumors in model groupⅠand Ⅲ was 6, while tumors in model Ⅱ group was about 2. In the three experimental groups the index of lung increased and the brain, spleen and thymus decreased compared with the control group. The bronchial alveolar lavage fluids analysis showed that the number of lymphocytes and polymorphonuclear leukocytes in the model group are more than that in control group.CONCLUSION All the three experimental groups could induce lung adenomas absolutely. Compared with model group Ⅱ, the model Ⅲ and Ⅰ are more stable and homogeneous with the similar results of experiment.

关 键 词：乌拉坦 C57BL/6J小鼠 肺腺瘤 动物模型 

分 类 号：R965[医药卫生—药理学]