PRSS23基因干扰与过表达载体构建及对牛MEC的影响  

Effects of interference and overexpression on MEC in cow

作　　者：李晓慧[1] 耿亚楠 姜平[1] 夏立新[1] 刘小川[1] 葛言亮 孙豪 杨润军[1] 赵志辉[1,2] LI Xiao-hui1 ,GENG Ya-nan1 ,JIANG Ping1 ,XIA Li-xin1 ,LIU Xiao-chuan1 ,GE Yan-liang1 ,SUN Hao1 , YANG Run-jun1 , ZHAO Zhi-hui1,2.(1. College of Animal Science, Jilin University, Jilin Changchun 130062, China ; 2. Agricultural College, Guangdong Ocean University, Zhanjiang, Guangdong 524088, Chin)

机构地区：[1]吉林大学动物科学学院,吉林长春130062 [2]广东海洋大学农学院,广东湛江524088

出　　处：《中国兽医学报》2018年第5期1008-1012,1034,共6页Chinese Journal of Veterinary Science

基　　金：国家“863”计划资助项目(2013AA102505);吉林省省级产业技术研究和发展项目(2016C032)

摘　　要：以奶牛乳腺上皮细胞为材料，在克隆PRSS23基因的基础上，构建真核过表达栽体和干扰栽体并将其转染到奶牛乳腺上皮细胞中，利用qRTPCR的方法检测PRSS23基因在mRNA水平上的表达情况，同时测定细胞内甘油三酯的含量。结果显示：在mRNA水平上，过表达组与对照组相比表达量差异显著，其甘油三酯水平显著升高；干扰组与对照组相比，在mRNA水平上差异表达显著，甘油三酯含量也显著升高。On the basis of cloning PRSS23 gene and using mammary gland epithelial cells as mate- rials,we transfected eukaryotic overexpression vector and interference vector which we constructed into mammary epithelial cells of dairy cow. The expression of PRSS23 gene at the mRNA level was detected by qRT-PCR and the intracellular triglyceride content was measured in cells. The results showed that the expression levels in the overexpression group compared with the control group were significantly different at the mRNA level, and the triglyceride level was significantly increased. The difference at mRNA level between the interference group and the control group was significant,and the triglyceride content was also significantly increased.

关 键 词：PRSS23 乳脂代谢 过表达载体 干扰载体 乳腺上皮细胞 

分 类 号：S814[农业科学—畜牧学] S852[农业科学—畜牧兽医]