雀儿舌头光谱性质及其与BSA相互作用的研究  

Spectral Properties of Leptopus Chinensis and Interaction between Leptopus Chinensis and Bovine Serum Albumin

作　　者：魏光璞 龙跃[1] WEI Guang-pu, LONG Yue(College of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou 450001, Chin)

机构地区：[1]郑州大学化学与分子工程学院

出　　处：《化学试剂》2018年第5期419-421,478,共4页Chemical Reagents

基　　金：国家自然科学基金资助项目(J1210060);河南省科技攻关计划资助项目(0624420031);河南省基础研究基金资助项目(022463001)

摘　　要：通过荧光光谱法和紫外吸收光谱法研究了中药雀儿舌头（Leptopus Chinensis,LC）的光谱性质以及LC与牛血清白蛋白（BSA）之间的相互作用。研究了溶剂、提取时间、浓度、pH对雀儿舌头提取物荧光特性的影响。乙醇作为提取溶剂,提取时间设定为10 min,在中性条件下测定。在激发波长为280 nm和狭缝宽度为5 nm条件下,观察到BSA的荧光猝灭以及LC的荧光敏化。LC对BSA中酪氨酸和色氨酸残基的影响结果表明,LC与BSA中色氨酸残基之间发生了能量转移。The spectroscopic properties of Leptopus Chinensis（LC） and the interaction between LC and bovine serum albumin（BSA） were investigated by fluorescence spectroscopy and UV absorption spectroscopy.The effects of solvent,extraction time,concentration,pH on the fluorescence properties of LC were investigated.The following experimental conditions were used finally：ethanol was selected as solvent,extraction time was set to 10 min,measured under neutral conditions. Fluorescence quenching of BSA was observed at an excitation wavelength of 280 nm and a slit width of 5 nm.BSA showed a fluorescence sensitization effect on LC.The effect of LC on the tyrosine and tryptophan residues of BSA showed that there was energy transfer between tryptophan residues in BSA and LC.

关 键 词：雀儿舌头 牛血清白蛋白 紫外吸收光谱 荧光光谱 同步荧光光谱 

分 类 号：R282.71[医药卫生—中药学]