共振光散射光谱法测定异烟肼的研究  

Determination of Isoniazid by Resonance Light Scattering Spectrometry

作　　者：朱梦[1,2] 李倩 梁艳[2,3] 马卫兴 ZHU Meng 1,2 ,LI Qian 1,2 ,LIANG Yan 2,3 ,MA Wei- xing 2,3(1.School of Chemical Engineering, 2. School of Pharmacy, 3.Jiangsu Key Laboratory of Marine Pharmaceutical School Screening, Huaihai Institute of Technology, Lianyungang 222005, Chin)

机构地区：[1]淮海工学院化学工程学院,江苏连云港222005 [2]淮海工学院药学院,江苏连云港222005 [3]淮海工学院江苏省海洋药物筛选重点实验室,江苏连云港222005

出　　处：《化学试剂》2018年第5期445-448,共4页Chemical Reagents

基　　金：淮海工学院研究生科研创新计划项目(XKYCXX2017-15)

摘　　要：在pH 4.10的B-R缓冲溶液中,利用异烟肼与过量的Fe^3＋先发生氧化还原反应,剩余的Fe3＋再与4-（2-吡啶偶氮）-间苯二酚（PAR）发生配位反应而生成配合物。异烟肼的加入使生成的配合物减少,从而引起体系的共振光散射信号显著减弱。在325 nm处减弱的共振光散射信号强度ΔI与浓度在0.05-0.30μg/mL范围内的异烟肼呈现良好的线性关系,由此建立了一种新的检测异烟肼含量的共振光散射光谱（RLS）分析方法。线性回归方程为ΔI=14 879.7c（μg/mL）＋448.6,相关系数（r）为0.999 6,检测限（L=3d/S）为0.013 5μg/mL。所拟方法成功应用于异烟肼片的测定,结果与药典法一致。In the B-R buffer solution at pH value of 4. 10,a novel resonance light scattering（RLS） analytical method of isoniazid was developed based on the redox reaction of isoniazid and excess Fe3＋,then the complex reaction of remaining Fe3＋with 4-（2-pyridylazo）-resorcinol（PAR） to form complexes.The addition of isoniazid reduced the formed complex,which resulting in a significant decrease of the resonance light scattering signal. The resonance light scattering signal intensity I weakened at 325 nm showed a good linear relationship with the concentration of isoniazid in the range of 0. 05 - 0. 30 μg/mL. The linear regression equation was ΔI = 14 879. 7 c（μg/mL） ＋448. 6.The correlation coefficient（r） was 0. 999 6.The limit of detection（L = 3 d/S） was0. 013 5 μg/mL.The proposed method has been applied to the determination of isoniazid tablets with satisfactory results,which are in agreement with those obtained by the pharmacopoeia method.

关 键 词：异烟肼 4-(2-吡啶偶氮)-间苯二酚 共振光散射光谱法 

分 类 号：O657.3[理学—分析化学]