长链非编码RNA-AK001903在原发性痛风患者中的变化及临床意义  

Expression and clinical significance of long noncoding RNA-AK001903 in patients with gouty arthritis

作　　者：姚承佼 蒲梦君[1] 青玉凤[2] 张全波[1] 文钟 周京国[3] Yao Chengjiao;Pu Mengjun;Qing Yufeng;Zhang Quanbo;Wen Zhong;Zhou Jingguo(Department of Geriatrics of the Affiliated Hospital, North Sichuan Medical College, Nanchong 637007, Chin)

机构地区：[1]川北医学院附属医学老年科,南充637007 [2]川北医学院附属医学风湿免疫科,南充637007 [3]成都医学院附属第一医院风湿免疫科

出　　处：《中华风湿病学杂志》2018年第5期323-327,共5页Chinese Journal of Rheumatology

基　　金：国家自然科学基金（81670801）.

摘　　要：目的探讨长链非编码RNA（lncRNA）AK001903在痛风性关节炎（GA）发病机制中的作用。方法将研究对象分为急性期组（AGA）30例，非急性期组（NAGA）24例、健康对照组24名、高尿酸血症组（HUA）24例。实时荧光定量多聚合酶链反应（RT-qPCR）检测AK001903在各组外周血单个核细胞（PBMCs）中转录水平表达，并分别与临床指标进行相关性分析；选取NAGA组12例和健康对照组12名，100 μg/ml尿酸盐晶体刺激全血后提取PBMCs检测AK001903转录表达水平。采用Kruskal-Wallis检验多组间比较，Mann-Whitney检验组间比较，Spearman相关分析行统计学处理。结果AK001903在AGA组（0.079±0.022）和NAGA组（0.071±0.021）的表达量均高于健康对照（0.014±0.004），AGA与NAGA组间差异无统计学意义（Z=-0.655，P〉0.05）；GA组（0.078±0.018）和HUA组（0.047±0.016）均高于HC组（0.014±0.004）（P〈0.05）；NAGA组、健康对照组外周血经尿酸盐刺激后AK001903的表达较空白对照组显著升高（Z=-2.887，Z=-4.157；P〈0.05）；AK001903转录水平与TG（r=0.938，P〈0.05）、VLDL（r=0.873，P〈0.05）呈正相关，与apoA1（r=-0.661，P〈0.05）呈负相关。 结论AK001903可能通过参与糖脂代谢与高尿酸血症的相互作用而影响痛风的发生发展。ObjectiveTo investigate the role of long noncoding RNA-AK001903 in the pathogenesis of primary gout arthritis（GA）.MethodsThe subjects were divided into four groups： 30 acute gout patients（AGA）, 24 non-acute gout patients（NAGA）, 24 healthy controls and 24 hyperuricemia（HUA）. Real-time quantitative polymerase chain reaction （RT-qPCR） was employed to examine the expression of AK001903 in peripheral blood mononuclear cells （PBMCs） from four groups. 100 μg/ml monosodium （MSU） was used to stimulate the peripheral blood of NAGA and healthy control patients. Then the expression of AK001903 was detected by RT-qPCR. Kruskal-Wallis test, Mann-Whitney test, Spearman correlations were used for statistical analysis.ResultsThe expression level of AK001903 in the AGA group （0.079±0.022） and the NAGA group （0.071±0.021） were higher than the healthy control group （0.014±0.004）. There was no significant difference between the NAGA group and the NAGA group （Z=-0.655, P〉0.05）. Those of the GA group （0.078±0.018） and the HUA group（0.047±0.016） was higher than the healthy control group （0.014±0.004） （Z=-2.887, Z=-4.157; P〈0.05）. Compared with the control group, the expression of AK001903 in NAGA and the healthy control group which were stimulated by MSU was significantly increased. The Spearman correlation analysis found that the AK001903 expression levels in the GA groups were correlated with TG （r=0.938, P〈0.05）, VLDL （r=0.873, P〈0.05）, GLU9 （r=0.671, P〈0.05） and were negatively correlated with apoA1 （r=-0.661, P〈0.05）.ConclusionAltered expression of AK001903 may be involved in the process of imbalance between lipid metabolism and hyperuricemia, and takes part in the pathogenesis of GA.

关 键 词：关节炎 痛风性 外周血单个核细胞 长链非编码RNA 尿酸盐 

分 类 号：R589.7[医药卫生—内分泌]