胀果甘草多糖对RAW264.7巨噬细胞免疫功能的影响  

Effects of Polysaccharide from Glycyrrhiza Inflata on Immune Function of RAW264.7 Macrophage Cell Line

作　　者：丛媛媛[1] 热米拉·米吉提 帕丽达·阿不力孜[1] 米仁沙·牙库甫[1] 陈橙[1] CONG Yuanyuan·Remila, Mijiti,Palida · Abulizi, Mirensha · Yakufu, CHEN Cheng(College of Pharmacy, Xinjiang Medical University, Urumqi 830011, Xinjiang, Chin)

机构地区：[1]新疆医科大学药学院

出　　处：《中华中医药学刊》2018年第5期1043-1047,共5页Chinese Archives of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(81360626);新疆维吾尔自治区高校科研计划重点项目(XJEDU2013I23)

摘　　要：目的：研究胀果甘草多糖GiP-B1对RAW 264.7巨噬细胞免疫功能的影响。方法：用浓度为25-400μg·mkg^-1的GiP-B1与RAW 264.7巨噬细胞共同培养,MTT法检测其细胞增殖率,中性红试验检测其吞噬功能,ELISA法检测其分泌TNF-α、IL-1β的功能,Griess法检测其释放NO和iNOS的能力,RT-q PCR法检测其iNOS、TNF-α、IL-1β的mRNA表达。结果：给药24 h后,与空白对照组相比,200-400μg·mkg^-1组的GiPB1可促进RAW264.7巨噬细胞的增殖,差异极显著（P〈0.01）;50-100μg·mkg^-1组的GiP-B1可显著促进RAW 264.7巨噬细胞的吞噬作用（P〈0.05）。作用RAW 264.7巨噬细胞48 h后,100μg·mkg^-1和400μg·mkg^-1组的GiP-B1可显著提高巨噬细胞的IL-1β分泌量（P〈0.05）,100-200μg·mkg^-1组的GiP-B1则可显著提高巨噬细胞分泌TNF-α的能力（P〈0.05）;不同浓度的GiP-B1干预RAW 264.7巨噬细胞后培养48 h,GiP-B1浓度增至100-400μg·mkg^-1时,NO生成量显著高于阴性对照组（P〈0.05）,且与GiP-B1浓度存在剂量效应关系;NOS生成量也高于阴性对照组,但仅200μg·mkg^-1组有显著性差异（P〈0.05）。给药24 h后,100μg·mkg^-1和400μg·mkg^-1组的GiP-B1可显著增加iNOS、TNF-α、IL-1β的mRNA表达量（P〈0.05）。结论：一定质量浓度的GiP-B1能增强RAW264.7巨噬细胞增殖率和吞噬能力,促TNF-α、IL-1β、NO及NOS释放,上调iNOS、TNF-α、IL-1β的mRNA水平,从而增强RAW264.7巨噬细胞的免疫功能。Objective： To study the effects of an acidic polysaccharide GiP-B1 from Glycyrrhiza inflata on immune function of RAW 264. 7 macrophage cell line. Methods： RAW 264. 7 macrophage cell line was as the research objects and different concentrations of GiP-B1（25 - 400 μg·mL^-1）were injected into RAW 264. 7 macrophage cell line. The blank control was without any treating. The effects of GiP-B1 on cell proliferation,phagocytosis of RAW 264. 7 cells,levels of NO,NOS,TNF-α and IL-1β production were detected by MTT assay,neutral red test,Griess method and ELISA method,respectively. The mRNA expression levels of TNF-α,IL-1β and iNOS were measured by RT-q PCR method. Results： Using different concentrations of GiP-B1 acting on RAW 264. 7 macrophage for 24 h,compared with the blank control group,certain concentrations of GiP-B1（200 - 400 μg·mL^-1）could promote the proliferation of macrophage and there were extremely significant differences（P〈0. 01）. GiP-B1 at the concentrations of 50 - 100 μg ·mL^-1 could significantly promote the phagocytosis of RAW 264. 7 macrophage（P〈0. 05）. After RWA 264. 7 macrophage was treated with different concentrations of GiP-B1 for 48 h,the production of IL-1β and TNF-α could significantly increased by GiP-B1 at the concentrations of 100 - 400 μg·mL^-1 and 100 - 200 μg·mL^-1,respectively（P〈0. 05）. Except the 25 - 50 μg·mL^-1 GiP-B1 groups,the production of NO was significantly higher than those of the negative control group（P〈0. 05）. The production of NOS was also higher than those of the negative control group,but there were no significant differences except the 200 μg·mL^-1 GiP-B1 group. The RT-q PCR experiments showed that the 100 μg·mL^-1 and 400 μg·mL^-1 GiP-B1 groups could significantly up-regulate the mRNA expressions of TNF-α,IL-1β and iNOS after treated for 24 h（P〈0. 05）. Conclusion： GiP-B1 at suitable concentrations can be contributed to the proliferation of the macrophages and the phagocytic activity,increase the pro

关 键 词：胀果甘草 多糖 巨噬细胞 免疫功能 

分 类 号：R285.5[医药卫生—中药学]